+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical analysis of transferrin receptor: Regional and cellular distribution in the hypotransferrinemic (hpx) mouse brain

, : Immunohistochemical analysis of transferrin receptor: Regional and cellular distribution in the hypotransferrinemic (hpx) mouse brain. Brain Research 801(1-2): 171-181

The hypotransferrinemic (hpx) mouse mutant produces <1% of the normal circulating level of transferrin (Tf). Heterozygote animals of this strain (hpx/+) have approximately 50% of normal plasma Tf levels. In this study we examine the cellular and regional distribution of Tf receptor (Tf-R) in the brain of wild type, hpx/+ and mutant (hpx/hpx) mice. Also, using slot-blot (immunoblot) analysis, we describe the relative amount of Tf-R in brain microvessels of hpx/+ animals compared with wild type. Tf-R was seen primarily in neurons throughout the brains of wild type, hpx/+ and hpx/hpx animals. Gray matter areas immunoreacted more robustly than white matter areas. Oligodendrocytes and third ventricle tanycytes, both of which we have previously described as iron-positive, did not immunoreact for Tf-R. Tf-R immunohistochemical reaction in wild type, hpx/+ and hpx/hpx brains appeared similar. Immunoblot analysis of isolated cortical microvessels from wild type and hpx/+ animals revealed no upregulation of Tf-R expression in hpx/+ (relative to normal) despite a 50% decrease in circulating Tf levels. These results indicate that Tf-R is primarily expressed by neurons and that half normal levels of Tf (hpx/+) or transferrin supplementation (hpx/hpx) are apparently sufficient for normal expression and distribution of Tf-R. Because of the lack of circulating Tf, but unaltered Tf-R expression, hpx mice could serve as a model for delivery of therapeutic agents via the Tf/Tf-R system.


PMID: 9729367

DOI: 10.1016/s0006-8993(98)00575-7

Other references

Antipov, V.G., 1986: Resistance of pine plantations to industrial gas emissions. A brief review of some effects of gases (mainly SO2) on characteristics such as needle development, increment and reproduction (pollen, seed development, seed quality). Data are presented on the effects of SO2 exposure (8 mg/msuperscript 3 for 1-2...

Pevetz, W., 1995: World population and world nutrition. OECD, FAO, UNDP, IRRI and other sources are reviewed to assess current trends in population growth and food supply. The problems are particularly acute in the Third World where it is estimated that some 800 million people are short of food and som...

Goldstein, N.S.; Ahmad, E.; Hussain, M.; Hankin, R.C.; Perez-Reyes, N., 1998: Endocervical glandular atypia: does a preneoplastic lesion of adenocarcinoma in situ exist?. Some believe endocervical glandular atypia (EGA), purportedly composed of cells that are less atypical than cells of adenocarcinoma in situ (AIS), is a preneoplastic precursor of AIS. We examined 246 neoplastic and nonneoplastic cervical cone biop...

Ferdinand, K.C., 2010: Management of high blood pressure in African Americans and the 2010 ISHIB consensus statement: meeting an unmet need. Journal of Clinical Hypertension 12(4): 237-239

Sagata N.; Yasunaga T.; Tsuzuku Kawamura J.; Ohnishi K.; Ogawa Y.; Ikawa Y., 1985: Complete nucleotide sequence of the genome of bovine leukemia virus its evolutionary relationship to other retroviruses. The complete 8714-nucleotide sequence of the integrated bovine leukemia virus genome was reported and the following genomic organization were deduced: 5' LTR[long terminal repeat]-gag-pol-env-pXBL-3' LTR, where LTR represent a long termi...

Habu, Y.; Yaegashi, M., 2001: Cardiovascular information measurement system. It is an object of the present invention to provide a cardiovascular information measurement system capable of performing an accurate blood pressure measurement and calculating an accurate cardiovascular index in consideration of the pulsation of...

Enblad, G.; Sundstrom, C.; Glimelius, B., 1993: Immunohistochemical characteristics of Hodgkin and Reed-Sternberg cells in relation to age and clinical outcome. One hundred and fifty-four cases of Hodgkin's disease diagnosed between 1985 and 1988 from an unselected population were stained with a panel of six monoclonal antibodies; LN1, MB2, L26 (CD20), all B-cell antibodies, UCHL1 (CD45 RO), mainly T...

Allen, G., 1890: The Taxidermist's Manual. Unknown

Newton, R., 1974: William Newton, 1893--1973. Phytopathology 64 (2) 160

Slattery, K.; Bentley, D.; Coutts, A.J., 2015: The role of oxidative, inflammatory and neuroendocrinological systems during exercise stress in athletes: implications of antioxidant supplementation on physiological adaptation during intensified physical training. During periods of intensified physical training, reactive oxygen species (ROS) release may exceed the protective capacity of the antioxidant system and lead to dysregulation within the inflammatory and neuroendocrinological systems. Consequently,...