geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical and biochemical indicators of muscle damage in vitro: the stability of control muscle and the effects of dinitrophenol and calcium ionophore


, : Immunohistochemical and biochemical indicators of muscle damage in vitro: the stability of control muscle and the effects of dinitrophenol and calcium ionophore. International Journal of Experimental Pathology 75(5): 329-343

The biochemical, histological and ultrastructural effects of 2,4-dinitrophenol and the calcium ionophore, A23187, on rat soleus muscle incubated in vitro have been examined to test the hypothesis that immunohistochemical techniques can be used to recognize early structural features of fibre damage. In control muscles, despite mild glycogen depletion and a mild reduction in protein synthetic rate in the central portion of the muscle, fibres throughout the muscle appear to be viable with normal cytoskeletal and contractile protein architecture, normal concentrations of high energy phosphates and no creatine kinase efflux. Dinitrophenol causes rapid creatine kinase efflux, extensive loss of immunolabelling for desmin and dystrophin, and abnormal myosin immunolabelling. Creatine kinase efflux and the changes in desmin and dystrophin are reduced by the exclusion of calcium. A23187 causes more gradual creatine kinase efflux associated with changes in myosin immunolabelling, but loss of desmin and dystrophin immunolabelling is restricted to a few of the most peripheral fibres. The results suggest that immunohistochemical methods can be used to reveal differences in the intracellular mechanisms of muscle damage. Although both dinitrophenol and A23187 may act, in part, through calcium-mediated processes, their effects on cytoskeletal proteins differ. Creatine kinase efflux after A23187 may not be due to gross sarcolemmal damage.

US$29.90

PMID: 7999634


Other references

Anonymous, 1984: Recent developments in the finance of agriculture. The article discusses agricultural credit in Italy, the institutions involved and their characteristics, the development of credit flows, public intervention and current problems and future outlook.

Corley, R.A.; Wilson, D.M.; Hard, G.C.; Stebbins, K.E.; Bartels, M.J.; Soelberg, J.J.; Dryzga, M.D.; Gingell, R.; McMartin, K.E.; Snellings, W.M., 2008: Dosimetry considerations in the enhanced sensitivity of male Wistar rats to chronic ethylene glycol-induced nephrotoxicity. Male Wistar rats have been shown to be the most sensitive sex, strain and species to ethylene glycol-induced nephrotoxicity in subchronic studies. A chronic toxicity and dosimetry study was therefore conducted in male Wistar rats administered ethy...

McCoy, C.J.; Smith, H.M.; Tihen, J.A., 1967: Natural hybrid toads, Bufo punctatus x Bufo woodhousei, from Colorado. Southwestern Naturalist, 12: 45-54

Yadav, D.V., 1993: Response behaviour of sugarcane to NPKS and micronutrients in different parts of the country. The yield responses of sugarcane to N, P, K, S and micronutrients in Maharashtra, Karnataka, Madhya Pradesh, Bihar, Uttar Pradesh, Punjab and Assam are reported. Each kg of soil applied N, P, K, NPK, S, Zn, Fe and Mn under irrigated conditions pro...

Fumelli, A., 1951: Let us prepare in time for the new wheat season. Italian varieties are recommended for growing in the plains of Italy and the following newer varieties are singled out as having given good results in preliminary trials and demonstration plots: Marchetti 102, Race B, Race C, Trento and Race P, br...

Shibata J.I., 1967: Hypno therapy of patients taking unbalanced diets human. American Journal of Clinical Hypnosis 10(2): 81-83

Raharivololoniaina, L.; Grosjean, S.; Raminosoa, N.R.soamampionona; Glaw, F.; Vences, M., 2006: Molecular identification, description, and phylogenetic implications of the tadpoles of 11 species of Malagasy treefrogs, genus Boophis. Based on specimens identified by DNA barcoding, we describe the tadpoles of 11 species of treefrogs (Boophis) in the Malagasy family Mantellidae. All tadpoles belong to species of the stream-breeding clade within Boophis. Based on these and other...

Paulauskis, J.D.; Sul, H.S., 1988: Cloning and expression of mouse fatty acid synthase and other specific mRNAs. Developmental and hormonal regulation in 3T3-L1 cells. Mouse liver mRNA enriched in sequence coding for fatty acid synthase by sucrose density gradient centrifugation was used as a template for cDNA synthesis. Double-stranded cDNA sequences were inserted into pBR322 and lambda gt10, and were cloned. C...

Pirmoradi, F.Nazly.; Jackson, J.K.; Burt, H.M.; Chiao, M., 2011: A magnetically controlled MEMS device for drug delivery: design, fabrication, and testing. We report the development of a magnetically controlled drug delivery device for on-demand drug release to treat chronic diseases. The devices consist of drug-loaded micro-reservoirs (6 mm in diameter and ∼550 μm in depth), sealed by magnetic PD...

Mastaglia, F.L.; Savas, S.; Kakulas, B.A.; Lekias, J.S., 1971: Thrombosis of the internal carotid artery after closed head injury. Proceedings of the Australian Association of Neurologists 8: 93-100