+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical and histochemical properties of surgically excised subretinal neovascular membranes in age-related macular degeneration

, : Immunohistochemical and histochemical properties of surgically excised subretinal neovascular membranes in age-related macular degeneration. American Journal of Ophthalmology 114(4): 464-472

The immunohistochemical and histochemical properties of 16 surgically excised subretinal neovascular membranes from 16 patients with age-related macular degeneration were studied. Primary antisera to c-retinaldehyde-binding protein; leukocyte common antigen; factor VIII-related antigen; S-100 protein; glial fibrillary acid protein; muscle-specific actin; neuron-specific enolase; collagen types I, II, III, IV, and V; laminin; and fibronectin were used for immunohistochemical characterization of the membranes. Histochemical staining for lipid and mucopolysaccharide was performed. The results of the staining in conjunction with histologic examination showed the cellular components of the membranes to be composed of retinal pigment epithelium, inflammatory cells, vascular endothelium, glial cells, myofibroblasts, photoreceptor cells, and fibrocytes. The extracellular matrix of the membranes contained collagen types I, III, IV, and V; fibronectin; laminin; mucopolysaccharide; and lipid. These findings are consistent with the concept that subretinal neovascular membranes in age-related macular degeneration are composed of localized intra-Bruch's membrane granulation tissue proliferation associated with diffuse drusen.


PMID: 1415458

DOI: 10.1016/s0002-9394(14)71859-8

Other references

Mishra, L.; Bankert, L.; Voyles, N.; Mishra, B.; Gearhart, J.; Mezey, E., 1993: Characterization of a novel stage-specific gene isolated from developing liver. Gastroenterology 104(4 SUPPL ): A956

Galadini, F.; Ceccaroni, E.; Falcucci, E., 2010: Archaeoseismological evidence of a disruptive Late Antique earthquake at Alba Fucens central Italy. Paleoseimological investigations in the 1990s identified a surface faulting event in the Fucino Plain (central Italy) related to the 5th-6th century AD. This event originated along the same seismogenic source responsible for the 1915 earthquake (M...

Achiwa, S., 1957: Studies on protective action of gelatinous matter in potentiometric titration. Generally, the greater the amount of gelatin added, the greater the deviation of apparent end points of the reaction from the theoretical point; the titratlon end point fell short of the theoretical value. Pb and Fe(CN)6 showed the largest deviati...

Gordon, G.H.; Baker, L.H.; Avalos, K.; Debar, L.; James, K.; Larsen, G.C., 1999: Predicting health care utilization in patients with chest pain Relative roles of psychiatric and coronary disease. JGIM 14(SUPPL 2): 35, April

Fitzpatrick, E.; Sullivan, J.; Smith, A.; Mucowski, D.; Hoffmann, E.; Dunn, P.; Trice, M.; Grosso, L., 1991: Clinical nursing research priorities: a Delphi study. Using the delphi technique, a group of clinical nurse specialists was surveyed regarding delineation of clinical nursing research priorities. Upon completion of the four-round survey, the results suggested that the top priorities for nursing resea...

Graves, S.C.; Dutkowsky, J.P.; Richardson, E.G., 1993: The Chevron bunionectomy: a trigonometric analysis to predict correction. A trigonometric analysis was developed in an attempt to predict the postoperative hallux valgus angles and intermetatarsal angles after Chevron bunionectomy. This analysis was used in 20 feet to predict postoperative angles, and these predicted va...

Ishikawa, T., 2013: Prognostic factors of myelodysplastic syndromes. Nihon Rinsho. Japanese Journal of Clinical Medicine 70 Suppl 2: 357-361

Kimura, K.A.; Reynolds, J.N.; Boegman, R.J.; Brien, J.F., 1998: Nitric oxide synthase enzymatic activity and protein expression increase throughout development of the guinea pig hippocampus. Society for Neuroscience Abstracts 24(1-2): 2059

Majstorovic, G.; Pantelic, M., 1972: Variations in the NPK content of the seed, stone and mesocarp of Pozegaca plums. Regular analyses from inception to maturity (20 September) of Pozegaca plums showed that the N content in the kernel varied little, in the endocarp it dropped suddenly and then disappeared, and in the pulp it fell gradually. The P2O5 content decre...

Kritzinger, S.M.; Kilian, S.G.; Potgieter, M.A.; Du-Preez, J.C., 2003: The effect of production parameters on the synthesis of the prebiotic trisaccharide, neokestose, by Xanthophyllomyces dendrorhous (Phaffia rhodozyma). The effects of important process parameters on the production of the novel prebiotic trisaccharide neokestose from sucrose by Xanthophyllomyces dendrorhous cells suspended in buffer were investigated. Recycling of cells reduced the level and rate...