+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical and in situ mRNA hybridisation techniques to determine the distribution of ion channels in human brain: A study of neuronal voltage-dependent calcium channels

, : Immunohistochemical and in situ mRNA hybridisation techniques to determine the distribution of ion channels in human brain: A study of neuronal voltage-dependent calcium channels. Brain Research Protocols 1(3): 299-306

The molecular, structural and functional characterization of ion channels in the CNS forms an area of intense investigation in current brain research. For strategic and logistical reasons, rodents have historically been the species of choice for these studies. The examination of human CNS tissues generally presents the investigator with specific challenges that are often less problematic in animal studies, e.g. postmortem delay/agonal status, and thus both the experimental design and techniques must be manipulated accordingly. Since much pharmaceutical interest is currently focused on neuronal ion channels, the examination of their expression in human brain material is of particular importance. We describe here the details of methods that we have developed and used successfully in the study of the expression of voltage-dependent calcium channels (VDCCs) in human CNS tissues (15). Presynaptic neuronal VDCCs control neurotransmitter release (11,8) and are important new drug targets (3). They are composed of three subunits, alpha1, beta and alpha2/delta (4) and multiple gene classes of each protein have been identified (7,12,14). Little is known, however, about the distribution of neuronal VDCCs in the human central nervous system, although initial studies have been performed in rat and rabbit (6,13,16).


PMID: 9385069

DOI: 10.1016/s1385-299x(97)00003-2

Other references

Lassus, H.; Leminen, A.; Lundin, J.; Lehtovirta, P.; Butzow, R., 2003: Distinct subtypes of serous ovarian carcinoma identified by p53 determination. Objective. The overall prognosis of ovarian carcinoma is poor. However, the outcome of apparently similar cases is highly variable, and molecular markers that would predict disease outcome in a clinically useful manner are lacking. We investigated...

Pinheiro,J.C.A.; Padua,L.E.deM.; Portela,G.L.F.; Branco,R.T.P.C.; Reis,A.S.dos; Silva,P.R.R., 2008: Biology comparison the Spodoptera frugiperda viewing the zoneament ecologic in Piaui State. Studied biology of <i>Spodoptera frugiperda</i> (J. E. Smith, 1797) in four constants temperatures (20 degrees , 25 degrees , 30 degrees , 32 degrees C) and with fotofase of twelve hours. With to determinate and goal termic constant of...

Rouge, P., 1981: Study of the immunological cross-reactions between lectins of legumes: systematic and phytogenetic implications. Seed extracts from 56 legume spp. including Phaseolus vulgaris, Vicia sativa, pea, lucerne, white clover and red clover were examined for immunological relatedness using 11 different lectin antisera. The immunological cross-reactions observed indi...

Mckenzie, J.M., 1964: Neonatal Graves' Disease. Journal of Clinical Endocrinology and Metabolism 24: 660-668

Pierson, G.P., 1975: Problems associated with importation of exotic birds. Proceedings, Annual Meeting of the United States Animal Health Association: 214-218

Slepko, Ii, 1978: Study of bending tensions in the carrying cable during the movement of a light cargo. Izvestiia vysshikh uchebnykh zavedenii Lesnoi zhurnal: ) 56-61

Wubet, T.; Weiss, M.; Kottke, I.; Oberwinkler, F., 2003: Morphology and molecular diversity of arbuscular mycorrhizal fungi in wild and cultivated yew (Taxus baccata). Taxus baccata roots collected from two sites in southern Germany were heavily colonized by arbuscular mycorrhizal fungi (AMF). The colonization pattern was of the Paris type. The diversity of the colonizing AMF species was investigated using polym...

Gerner, E.W.; Leith, J.T., 1977: Interaction of hyper thermia with radiations of different linear energy transfer. To determine how hyperthermia interacts with radiation as a function of LET [linear energy transfer], single-cell survival parameters were measured in Chinese hamster ovary [CHO] cells after exposure to accelerated carbon ions (LET = 34.1 keV/.mu....

Chester, A.; Chester, J., 1985: Emergency medicine: viral hepatitis type B vaccine. Western Journal of Medicine 142(3): 384-385

Pardal, M.M.; Micheli, F.; Asconapé, J.; Paradiso, G., 1985: Neurobehavioral symptoms in caudate hemorrhage: two cases. Neurology 35(12): 1806-1807