geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical and ultrastructural localization of CGRP-positive nerve fibers at the epiphyseal trabecules facing the growth plate of rat femurs


, : Immunohistochemical and ultrastructural localization of CGRP-positive nerve fibers at the epiphyseal trabecules facing the growth plate of rat femurs. Bone (new York). 18(1): 29-39

We performed immunohistochemical and ultrastructural studies to disclose a possible relationship between nerve fibers and bone metabolism. Immunohistochemical distribution of calcitonin gene-related peptide (CGRP)-positive nerve fibers during bone development was assessed in the femurs of rats. CGRP-positive nerve fibers were denser in the epiphysis than in the metaphysis. These nerve fibers particularly ran along the epiphyseal trabecules facing the growth plate and came in contact with osteoclasts. Many osteoclasts at the epiphyseal trabecules facing the growth plate contained abundant toluidine blue and periodic acid-Schiff-positive granules. Electron microscopy revealed that these osteoclasts have many membrane-bound, electron-dense granular structures and dilated cisterns of rough endoplasmic reticulum containing electron-dense material. They were often surrounded by clear cells displaying features of nerve fiber and had no ruffled border. Furthermore, ultrastructural observations revealed electron-dense structures coating the cytoplasmic side of plasma membranes of the nerve fibers. We also observed coated pits in the cytoplasm of the osteoclasts facing the nerve fibers. To further clarify the role of innervation, we compared trabecules of rats undergoing denervation of the sciatic nerve with those from unoperated rats. Denervation resulted in a significant increase in the number of cement lines on the epiphyseal trabecules facing the growth plate. These results suggest that the osteoclasts at the epiphyseal trabecules facing the growth plate are in part regulated by CGRP-positive nerve fibers. Thus, CGRP-positive nerve fibers could be a crucial element in bone metabolism during bone growth and development.

US$19.90

PMID: 8717534

DOI: 10.1016/8756-3282(95)00425-4


Other references

Yu. G.Trofimenkov, 1990: International recommendations on penetration testing of soils. Soil Mechanics and Foundation Engineering 27(3): 119-120

Tu, C.C.; Cheng, Y.H.; Wang, C.Y., 1976: Screening of flax lines resistant to Fusarium oxysporum. Mass selection of 23 out of 120 lines already screened for resistance to Fusarium oxysporum resulted in lines with only 5% disease incidence and with the agronomic characteristics of Tai-chung-hsuan 1 [Taichung Selection 1].

Blake Coleman B.C.; Cossar J.D.; Clarke D.J.; Ramsay C., 1990: Oscillometric instrument for the non invasive detection of low level microbial activity part 1 apparatus. A significant improvement in the design of the oscillometer and its use as an analytical instrument for microbial, conductimetric and enzyme evaluations is reported. Employing a novel hybrid test cell and specialised amplifer circuits, a very high...

Brockman, A., 1970: Experiences with orchard soil. Spring 34 2-4

Woods N.F., 1982: Menopausal distress a model for epidemiologic investigation. Voda, A M , M Dinnerstein And S R O'donnell (Ed ) Changing Perspectives on Menopause Xix+404p University Of Texas Press: Austin, Texas, Usa Illus P220-238

Tye, H., 1987: Breeding biology of Picathartes oreas. Gerfaut, 773: 313-332

Kaplan L.D.; Northfelt D.; Kahn J.; Volberding P., 1992: Degree of immunodeficiency at diagnosis and treatment outcome in hiv associated non hodgkins lymphoma hiv nhl. Viii International Conference on Aids And The Iii Std World Congress Viii International Conference on Aids And The Iii Std World Congress; Harvard-Amsterdam Conference, Amsterdam, Netherlands, July 19-24, Pagination Varies Viii International Conference on Aids And The Iii Std World Congress: Amsterdam, Netherlands Paper : b105

Ersbøll, J., 1989: Remission with antineoplastic agents?. Ugeskrift for Laeger 151(29): 1885-1886

Montaser, R.; Abboud, K.A.; Paul, V.J.; Luesch, H., 2011: Pitiprolamide, a proline-rich dolastatin 16 analogue from the marine cyanobacterium Lyngbya majuscula from Guam. An unusual cyclic depsipeptide, pitiprolamide (1), was isolated from the marine cyanobacterium Lyngbya majuscula collected at Piti Bomb Holes, Guam. The structure was deduced using NMR, MS, X-ray crystallography, and enantioselective HPLC-MS techn...

Lewis, J.H.; Collier, K.P.; Marino, G.A.; Epstein, M.S.; Howell, C.D.; Thuluvath, P.; Ravendhran, N.; Cox, M.; Schulman, A.; Dellon, S.; Finkel, R.; Lobis, I.; Koch, M.; Julie, N.; Sato, T.; Gelfand, R.; Korman, L.; Chasen, R.; Musselman, R.; Diamond, A.; Golding, M.; Doman, D.; Hunt,.;, 2000: Lack of difference in response to Rebetron by race in prior interferon non-responders with chronic hepatitis C. American Journal of Gastroenterology 95(9): 2508-2509, September