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Immunohistochemical assessment of human microsomal epoxide hydrolase in primary and secondary liver neoplasm: a quantitative approach

, : Immunohistochemical assessment of human microsomal epoxide hydrolase in primary and secondary liver neoplasm: a quantitative approach. Xenobiotica; the Fate of Foreign Compounds in Biological Systems 26(1): 107-116

1. Epoxide hydrolases form an enzyme family involved in the metabolism of a variety of xenobiotics including cytostatic drugs and carcinogens. Whether human microsomal epoxide hydrolase--one of the main members of the epoxide hydrolase family--is expressed in neoplasia of the liver has been the subject of a controversial discussion. 2. We therefore developed a quantitative immunohistochemical assay and monitored epoxide hydrolase expression in hepatocellular carcinomas (HCC, n = 20), cholangio-cellular carcinomas (CCC, n = 2) and liver metastases (n = 57) of tumours of various origins, and compared the expression intensities and patterns to normal liver tissue. 3. In normal liver tissue microsomal epoxide hydrolase displays expression of the constitutive type with non-zonal staining of all hepatocytes. 4. When using a quantitative immunohistochemical approach statistically significant differences in microsomal epoxide hydrolase expression were observed between normal tissue, hepatocellular carcinoma and liver metastases (mean optical density 2.35, 1.63 and 0.21 respectively, p = 2.9, 6.3 and 18.9). These data indicate differential expression in different types of liver neoplasm. 5. As microsomal epoxide hydrolase is involved in metabolism of different xenobiotics our findings may have implications for tumour progression.


PMID: 8851825

DOI: 10.3109/00498259609046692

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Ravishankar, N.; Bernstein, M., 2016: Religion benefiting brain tumour patients: a qualitative study. As the focus on modern neurosurgery has shifted to the realm of technological advancement, some patients and their loved ones still hold a strong faith in their religion to guide them through the process. This study aimed to determine whether reli...

Tomas, J., 1979: Effect of various doses of sulfur on the resorption of phosphorus and potassium by alfalfa in different soil types. Pol'nohospodarstvo = Sel'skoe khoziaistvo = Agriculture5(3): 231-241

Sarna, S.; Hoppu, K.; Neuvonen, P.J.; Laine, J.; Holmberg, C., 1997: Methylprednisolone exposure, rather than dose, predicts adrenal suppression and growth inhibition in children with liver and renal transplants. Some patients receiving glucocorticoids develop adverse effects even with very low doses, whereas others fail to achieve the desired effects with the usual therapeutic doses. We hypothesized that glucocorticoid exposure, rather than the dose, woul...

Sokhey, S.S.; Habbu, M.K., 1950: Casein hydrolysate cholera vaccine. Bulletin of the World Health Organization 3(1): 33-42

Liu, Y.; Sun, J.; Wu, C.; Cao, X.; He, M.; You, C., 2012: The interleukin-6-572G/C gene polymorphism and the risk of intracranial aneurysms in a Chinese population. Interleukin-6 (IL-6) is an important proinflammatory cytokine that plays an important role in the pathogenesis of intracranial aneurysms (IAs). The aim of this study was to investigate the association between the IL-6-572G/C polymorphism and the r...

Diribe, C.O.; Warhurst, D.C., 1985: A study of the uptake of chloroquine in malaria-infected erythrocytes. High and low affinity uptake and the influence of glucose and its analogues. A study of concentration- and substrate-dependence of chloroquine uptake has been carried out on mouse erythrocytes infected with the chloroquine-sensitive NK65 and the chloroquine-resistant RC strains of Plasmodium berghei. The presence of drug b...

Sykes, L.R.; Moran, S. ; Beeler, N. ; Wong, I. ; Weldon, R. ; McConnell, V. ; Trehu, A., 2010: Seismological monitoring of the Comprehensive Nuclear Test Ban Treaty. Seismological Research Letters 81.2

Vasiljeva, II.; Pshennikova, EV., 1996: Algae of the Kolyma River and water bodies of its basin Russia. Al'gologiya, 61: 35-41, 102

Anonymous, 1978: Robert I. Papendick. Agronomy Journal: 70 (1) 154

Gorham, J., 1980: The stilbenoids. This review covers the distribution of naturally occurring stilbenoids and their detection, identification, biosynthesis and physiological functions.