geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical assessment of proliferative activity in adrenocortical neoplasms


, : Immunohistochemical assessment of proliferative activity in adrenocortical neoplasms. Modern Pathology 6(6): 663-668

Although many histologic criteria have been utilized to help distinguish benign from malignant adrenocortical tumors, it still may be difficult to assess the biologic potential of a given tumor. We evaluated 19 adenomas and 15 primary carcinomas with the avidin-biotin complex peroxidase method utilizing formalin-fixed, paraffin-embedded tissues with monoclonal antibodies for proliferating cell nuclear antigen (PC10) and Ki-67 (MIB 1) to determine if staining for these antigens could be used to help differentiate benign from malignant adrenocortical neoplasms. We also evaluated whether these markers could be used as prognostic indicators. Labeling indices for both PCNA and Ki-67 were determined by enumerating 1000 tumor cells, and expressed as a percentage of cells with nuclear staining. A PCNA and a Ki-67 score was obtained by the product of the staining intensity (0-3+) and the extent of nuclear staining, expressed as an estimate of the percentage of cells staining. Both PCNA and Ki-67 score and labeling index were correlated with mitotic counts, histologic diagnosis, and clinical outcome. Follow-up period for patients ranged from 4 months to 12 years with a mean of 25 months. Mitotic counts correlated with histologic diagnosis and clinical outcome. Both Ki-67 score and labeling index were significantly higher in malignant than in benign tumors, and correlated with mitotic counts and clinical outcome. There was a strong correlation between Ki-67 score and labeling index, indicating that Ki-67 score may be a more rapid and equally accurate method of estimating proliferative index of a tumor. PCNA score and labeling index did not correlate with histologic diagnosis or clinical outcome. These results indicate that monoclonal antibody MIB 1(Ki-67) can aid in the distinction between adrenocortical adenomas and carcinomas in routinely processed formalin-fixed, paraffin-embedded tissue sections.

US$29.90

PMID: 7508113


Other references

Olmi, P.; Scorsetti, M.; Brusa, A.; Mongioj, V., 2003: From clinical target volume to biological target volume. The authors' experience with a point matching algorithm for image registration belonging to a commercially available software package for conformal radiotherapy, is reported. The algorithm IFS (Image Fusion System) permits the registration of...

Amaratunga, K.; Goodwin, P.M.; O'Connor, C.D.; Anthony, C., 1997: Erratum to "The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens" [FEMS Microbiol. Lett. 146 (1997) 31-38. Fems Microbiology Letters 150(1): 175-177

Halbeisen, W.A.; Gruber, C.M.; Gruber, C.M., 1948: A toxicologic and pharmacologic investigation of cycloheptenylethyl barbituric acid. The LD50 of cycloheptenylethyl barbituric acid (Medomin) injected intraperit. was 284 mg./kg. in white mice and 220 mg./kg. in albino rats. Intraven. the LD50 was 119 mg./kg. in rabbits and 105 mg./kg. in dogs. Using duration of sleep as the crite...

Pan, W., 2006: Incorporating gene functions as priors in model-based clustering of microarray gene expression data. Motivation: Cluster analysis of gene expression profiles has been widely applied to clustering genes for gene function discovery. Many approaches have been proposed. The rationale is that the genes with the same biological function or involved in...

Parks, M.M., 1956: Strabismus; review of the literature for 1955. A.M.A. Archives of Ophthalmology 56(1): 138-159

Chetkina, N., 1988: A review of dairy breeds at the VDNKh, USSR. An exhibition entitled 'High-yielding breeds and new types of dairy cattle for progressive technology' held at the All-Union Exhibition of Economic Achievements (VDNKh) from July to Oct. 1987 is reviewed.

Socher, E.; Imperiali, B., 2013: FRET-capture: a sensitive method for the detection of dynamic protein interactions. Caught in the act: The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional l...

Snow, N.; Bergin, K.T.; Horrigan, T.P., 1985: Readmission of patients to the surgical intensive care unit: patient profiles and possibilities for prevention. Because experience is lacking regarding the profile of patients readmitted to a surgical ICU (SICU), we retrospectively reviewed total admissions, readmissions, patient profiles, and characteristics of illness requiring readmission to a multidisci...

Zaror, L.; Gutierrez, M.A.; Saez, F.N., 1982: Sensitivity in vitro of dermatophytes, Candida and other fungi to natamycin. All strs. of Microsporum canis were inhibited by 25 mu g or less and 93.7% by 12.5 mu g of natamycin; 84.1% of Trichophyton mentagrophytes strs. by 25 mu g and all T. rubrum strs. by 25 mu g or less; 85% of Candida albicans strs. by 6.25 mu g and...

Rohdendorf, B.B., 1980: The historical development of the Insecta. General regularities of insect evolution.. Trudy Paleontologicheskogo Instituta 175: 174-188