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Immunohistochemical assessment of tumor proliferation and volume of embryonal carcinoma identify patients with clinical stage A nonseminomatous testicular germ cell tumor at low risk for occult metastasis

, : Immunohistochemical assessment of tumor proliferation and volume of embryonal carcinoma identify patients with clinical stage A nonseminomatous testicular germ cell tumor at low risk for occult metastasis. Cancer (philadelphia). 75(3): 844-850

Background: Thirty percent of patients with clinical Stage A nonseminomatous testicular germ cell tumor (NSGCT) are incorrectly clinically staged. In a previous retrospective study at Indiana University, the combination of tumor proliferation rates by flow cytometry and histopathologic evaluation defined risk groups for occult metastatic disease in these patients with clinical Stage A NSGCT. A new immunohistochemical proliferation marker (MIB-1) was therefore used to assess growth fraction in combination with histopathology in an effort to predict pathologic stage in patients with clinical Stage A NSGCT. Methods: Primary orchiectomy specimens from 90 consecutive patients with clinical Stage A NSGCT (January 1992-November 1993) who underwent retroperitoneal lymph node dissection at Indiana University were histopathologically evaluated. Formalin fixed, paraffin embedded tissue sections were immunohistochemically stained using a monoclonal antibody against the nuclear proliferation-associated antigen Ki-67 (MIB-1). Satisfactory staining was obtained by using an antigen retrieval method based on microwave oven heating of paraffin sections. Results. MIB-1 immunohistochemical staining showed significant differences in mean values between 65 patients (66.1%) with pathologic Stage A NSGCT and 25 (80.4%) patients with pathologic Stage B NSGCT (P = 0.0032). The negative predictive value for patients with pathologic Stage A disease was 87% using a cut-off of 80% or less MIB-1 positively stained cells. A combined approach, using the absolute volume of embryonal carcinoma per patient ( lt 2 ml) and MIB-1 immunostaining ( lt 80%) was able to define a group of 30% of all patients who were at extremely low risk for occult metastatic disease. Conclusions: MIB-1 immunostaining in combination with histopathology aided in defining a low risk group patients with clinical Stage A NSGCT but failed to identify patients at high risk for metastasis. The risk factors need to be tested in a prospective clinical trial to determine if they are potentially useful in assigning therapy to individual patients.


PMID: 7828136

DOI: 10.1002/1097-0142(19950201)75:3<844::aid-cncr2820750316>;2-c

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Walhout, R.J.; Lekkerkerker, J.C.; Oron, G.H.; Bennink, G.B.W.E.; Meijboom, E.J., 2004: Comparison of surgical repair with balloon angioplasty for native coarctation in patients from three months to 16 years of age. Journal of the American College of Cardiology 43(5 Supplement A): 382A, March 3

Hermanowska-Szpakowicz, T.; Pancewicz, S.; Szulc, A.; Popławska, R., 1997: Mental disorders in patients after tick-borne encephalitis (TBE). There were 58 patients (aged 17 to 72) analyzed in our study: 34 women and 24 men one year after TBE. Psychic state was estimated with the use of psycho-pathologic scales CPRS, Hamilton Depression Rating Scale and Hamilton Anxiety Rating Scale, Mi...

Kirk, D.H.lmes, A., 1975: The heating of foodstuffs in a microwave oven. Journal of food technology: 10 (4) 375-384

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Mendes, M. de L.; Pria, M.D.; Geus Bouwman, D.M.; Vicente, F.R.; Tomaselli, F., 2001: Occurrence of Pratylenchus penetrans (Cobb, 1917) Chitwood & Oteifa, 1952, in Arracacia xanthorrhiza Bancr. at Castro, State of Parana, Brazil. P. penetrans is reported as the agent of brown lesions on Peruvian carrot (A. xanthorrhiza) roots in Castro, State of Parana, Brazil. The diseased tuberous roots showed small lesions, but sometimes these lesions were large, covering almost all the...

Frederick, G.L., 1960: A relationship between vitamin B12 and reproduction, and a method of diagnosing vitamin B12 deficiency in individual swine. Twelve fertile and 10 barren sows were given 250 [mu]g of vitamin B12 intramuscularly. Blood samples were taken prior to injection and 24- and 48-hours after injections. Serums were assayed for vitamin B12. It appeared that there was a relationshi...

Kuosa, M.; Haario, H.; Kallas, J., 2005: Axial dispersion model for estimation of ozone self-decomposition. A new method using the axial dispersion model for estimation of ozone se self-decomposition kinetics in a semibatch bubble column reactor was developed. The reaction rate coefficients for literature equations Of ozone decomposition and the gas pha...

Clauzade, G.; Roux, C., 1975: Lichens collected by jean amic in norway and great britain. Collection trips, authors, stations of different altitudes and 57 spp. comprising 14 genera of lichens are mentioned Lecanora subcarnea Kaj and Lecidea cyathoides are abundant.

Sinoquet, H.; Bonhomme, R., 1989: Modelling the interception of solar radiation in row crops. II. Crop geometry and validation of the model. A radiative transfer model was validated using a maize crop grown in Guadeloupe in rows oriented E.-W. or N.-S. Crop geometry as measured by the silhouette method varied with row orientation. The azimuthal distribution of the crop with E.-W. orien...

Subba Rao N., 1993: Environmental impact of industrial effluents in groundwater regime of Visakhapatnam industrial complex. Indian Journal of Geology: , Pages 35-43. 1993.