+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical characterization of 22 monoclonal antibodies against the CA125 antigen: 2nd report from the ISOBM TD-1 Workshop

, : Immunohistochemical characterization of 22 monoclonal antibodies against the CA125 antigen: 2nd report from the ISOBM TD-1 Workshop. Tumour Biology 17(6): 325-331

We evaluated the immunohistological (IH) characteristics of 22 different antibodies that were submitted for study in the frame of the TD-1 ISOBM Workshop on monoclonal antibodies against CA125. Information on relative affinities and epitope similarities was obtained from a parallel immunochemical study. Antibodies were tested at concentrations of 10 and 1 micrograms/ml on frozen and paraffin sections. Paraffin sections were stained according to the streptavidin-biotin complex protocol, and frozen sections according to a two-step immunoperoxidase technique. Aminoethylcarbazole served as the chromogen. The tissues were from normal proliferative endometrium (formalin-fixed paraffin-embedded) material and clear-cell adenocarcinoma of the ovary (formalin-fixed paraffin-embedded and frozen material). Sections were scored for staining in epithelial cells, basal, apical and diffuse cytoplasmic and in stromal components. Intensity was graded as 1, 2 or 3 for epithelial cells and as -1, -2 or -3 for stroma. The cumulative scores for each antibody expressed the discriminative properties of specific epithelial staining against background. M11 and M11-like antibodies, as well as OC125 and OC125-like antibodies, in general showed good staining results. Although there was a trend for high-affinity antibodies to show higher scores, there was no clear relationship between affinity and staining result. For nine antibodies (ZR45, MA602-1, K102, K94, K90, OV185, K97, K96, OV198), the reactions in paraffin and frozen sections were of similar intensity. Most of these were of low affinity with one exception: antibody ZR45, a rat monoclonal antibody (MAb) which had a high relative affinity. For eight antibodies (M11, K101, MA602-6, ZS33, B27.1, B43.13, K93, OC125), a loss of specific staining was observed in frozen sections. All but two of these antibodies (MA602-6 and OC125) were of high relative affinity. With four antibodies (K91, ZR38, K95, K100), the reverse situation was observed. One (K100) was of low affinity, two (K95 and K91) of high affinity and the fourth (ZR38) was a rat MAb of high affinity. Mainly due to the increased cytoplasmic staining in carcinoma, the reactivity in paraffin sections was less extensive in normal endometrium compared to ovarian carcinoma for the majority of antibodies, irrespective of their affinity or epitope group. The IH characterization of these antibodies may be of help in selecting antibodies with specific properties for further comparative studies. The reactivity of normal endometrium with all useful antibodies makes it a good candidate for standard external IH tissue control.


PMID: 8938947

DOI: 10.1159/000217996

Other references

Burnham Clifford Wayne; Tuttle Orville Frank, 1960: Composition of the magmatic vapor phase. Geological Society of America Bulletin 71(12, Part 2): 1837

Zhang, P.; Wang, T.; Qian, G.; Wu, D.; Frost, R.L., 2014: Removal of methyl orange from aqueous solutions through adsorption by calcium aluminate hydrates. Methyl orange (MO) is a kind of anionic dye and widely used in industry. In this study, tricalcium aluminate hydrates (Ca-Al-LDHs) are used as an adsorbent to remove methyl orange (MO) from aqueous solutions. The resulting products were studied by...

Ouyang, H.; Vogel, H., J., 1998: Melatonin and serotonin interactions with calmodulin: NMR, spectroscopic and biochemical studies. It has been reported that the hormone melatonin binds tightly to the ubiquitous calcium-regulatory protein, calmodulin (CaM) with a Kd value around 0.1 nM [Benítez-King et al., Biochim. Biophys. Acta, 1290 (1993) 191-196]. Normally CaM only binds...

Mazaredo, A.; Vergara, B.S., 1977: Screening for rapid elongation ability in small water tank. A method using a 100-cm deep water tank for the screening of 573 rice cv. for rapid elongation ability is presented. 39-day-old rice seedlings raised from seed in pots in a glasshouse were transferred to tanks in which the water depth was progress...

Bertrand, R.; Kohn, K.W.; Solary, E.; Pommier, Y., 1994: Detection of apoptosis-asociated DNA fragmentation using a rapid quantitative filter binding assay. Proceedings of the American Association for Cancer Research Annual Meeting 35(0): 312

Joseph Doetsch; Niklas Linde; Mirco Pessognelli; Alan G.G.een; Thomas Günther, 2012: Constraining 3-D electrical resistance tomography with GPR reflection data for improved aquifer characterization. Surface-based ground penetrating radar (GPR) and electrical resistance tomography (ERT) are common tools for aquifer characterization, because both methods provide data that are sensitive to hydrogeologically relevant quantities. To retrieve bulk...

Surdeanu, M.; Shundi, L.; Straut, M., 2000: Molecular subtyping of group A streptococcal strains based on virulence regulon polymorphism. A total of 64 Streptococcus pyogenes isolates included in a previous study (Shundi et al., 2000) were further analysed by Vir typing. Vir typing is based on restriction fragment length polymorphism (RFLP) analysis of a 4- to 8-kb pathogenicity isl...

Rant, J., 1954: Diagnosis and treatment of progenia in deciduous teeth. Zobozdravstveni Vestnik 9(1-2): 47-53

O'Sullivan, J.; Hasan, A.; Mitchell, L.; Hamilton, L.; Dark, J.H., 1997: Differential pulmonary flow after heart transplantation in patients with malposition of the great arteries. In transposition of the great arteries the right lung is preferentially perfused, and this may be due to the orientation of the right pulmonary artery when the main pulmonary artery comes off in a posterior position. In this study the mean perfusi...

Pioli, C.; Gatta, L.; Ubaldi, V.; Doria, G., 2000: Inhibition of IgG1 and IgE production by stimulation of the B cell CTLA-4 receptor. Although a large amount of information is available on the activity of CTLA-4 in T cells, the role of this receptor in B cells has not been previously characterized. Our results show that CD40 or LPS stimulation in the presence of IL-4 induces CTL...