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Immunohistochemical characterization of a stage-specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody


, : Immunohistochemical characterization of a stage-specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody. Cell Biology International 18(8): 819-827

In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carried out. The distribution of the delectable molecule with MAb X-80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X-80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X-80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.

US$19.90

PMID: 7804159

DOI: 10.1006/cbir.1994.1115


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