+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical characterization of subependymal giant cell astrocytomas

, : Immunohistochemical characterization of subependymal giant cell astrocytomas. Acta Neuropathologica. 91(4): 368-375

Subependymal giant cell astrocytoma (SEGA) is the most common neoplastic process involving the brain in patients with tuberous sclerosis complex (TSC). Morphologically, these tumors exhibit a wide range of cytoarchitecture with spindle and epithelioid cells resembling astrocytes, and also large, occasionally giant cells, some of which have a distinctly ganglion-like appearance. Unresolved questions regarding SEGAs center on: (a) their cytogenesis, i.e., whether they are derived from single or multiple precursors; and (b) their differentiating capacity along glial or neuronal lines. We sought to determine whether SEGAs represent truly mixed tumors or whether they consist of a single population of cells with a capacity for divergent differentiation. Twenty SEGAs were assessed for immunophenotypic features of either neuronal or glial differentiation or both. Only tumors from patients with a clinically confirmed diagnosis of TSC were included. Immunoreactivity for glial fibrillary acidic protein (GFAP) and/or S-100 protein was considered indicative of a glial phenotype, whereas the presence of neuronal differentiation was assessed by staining for cytoskeletal proteins (neurofilament epitopes, class III beta-tubulin, microtubule-associated protein 2 (MAP2), synaptophysin), neurosecretory substances (serotonin, cholecystokinin, beta-endorphin, substance P, somatostatin, metenkephalin, neuropeptide Y, vasoactive intestinal poly-peptide (VIP)), and for the 28-kDa neuron-associated calcium binding protein calbindin. Of the tumors examined, 18 exhibited both glial and neuronal epitopes, the staining pattern being variable. In 19 tumors, the constituent spindle, polygonal and giant or ganglion-like cells showed variable immunoreactivity for GFAP and S-100 protein, both within the cell body and processes. Neuron-associated cytoskeletal proteins were present in 18 cases. Class III beta-tubulin immunoreactivity was demonstrated in 17 tumors, both within the bodies of all three cell types and to varying degrees within their processes. Neurofilament protein and calbindin staining was present in 8 tumors, with reactivity for the former being distributed in a phosphorylation-dependent manner. MAP2 was detected in a few cells of two tumors. Immunoreactivity for neuropeptides was observed in 17 lesions. Somatostatin and met-enkephalin staining was noted in 10 tumors (50%), being present particularly within polygonal cells. Neuropeptide Y, scrotonin and beta-endorphin reactivity was found in 6 (30%), 5 (25%), and 4 tumors (20%), respectively; beta-endorphin was lacking in giant cells, whereas neuropeptide Y and serotonin were seen within their cell bodies. Substance P and VIP were evident in only occasional polygonal cells of 2 (10%) and 1 tumor (5%), respectively. Stains for cholecystokinin were negative. The observation of immunoreactivity for both glial- and neuron-associated epitopes within tumor cells of the same morphology suggests that SEGAs represent proliferations of cell lineages with the capacity to undergo divergent glioneuronal as well as neuroendocrine differentiation to a greater extent than do other mixed glial-neuronal neoplasms.


PMID: 8928613

DOI: 10.1007/s004010050438

Other references

Boslough John, 1981: The tortuous course of the Colorado. Science (Washington, 1979) 2(5): 30-37

Varani, J.; Fligiel, S.E.; Till, G.O.; Kunkel, R.G.; Ryan, U.S.; Ward, P.A., 1985: Pulmonary endothelial cell killing by human neutrophils. Possible involvement of hydroxyl radical. Human blood neutrophils stimulated by a variety of agents were shown to have cytotoxic effects on bovine pulmonary artery endothelial cells. Effective agonists included immune complexes, opsonized zymosan and 12-O-tetradecanoyl phorbol acetate. Un...

Pallarito, K., 1992: Bankruptcy looms for hospital defendant. Modern Healthcare 22(3): 16-16

Bonn G.K.; Kalghatgi K.; Horne W.C.; Horvath C., 1990: Rapid metal interaction chromatography of proteins and peptides on micropellicular sorbents. Chromatographia 30(9-10): 484-488

Tanaka, S.; Takeoka, M., 1972: On the fertility of the poultry manure for forest fertilizer. Tests in plots of newly planted Cryptomeria japonica trees showed that poultry manure had as beneficial an effect on d.b.h. and height growth as NPK fertilizer. Trials of the practical use of poultry manure as a forest fertilizer remain to be made...

Neander W.L.; Morse J.M., 1989: Tradition and change in the northern alberta canada woodlands cree implications for infant feeding practices. Using anthropological, ethnographic methods of participants observation and interactive, unstructured interviews, we compared traditional and present-day infant feeding practices of the Northern Alberta Wodland Cree. Twelve elderly multigravidae w...

Tsanev K., 1986: Early invasive forms of uterocervical cancer classification and biological characteristics. The author reports his studies on the possibility for metastases of the early invasion forms of the uterine neck cancer according to the extent of their invasion capacity, measured in micrones. On the basis of the obtained results the author deter...

Kimata, I.; Yashima, S.; Yamazaki, T.; Yuen, C.K., 1983: Analysis of runoff and consumptive use (evapotranspiration) in paddy field using water balance before tertiary canal construction in the Muda Area, Malaysia. Data are presented on the water balance, runoff and consumptive use of water in one of the blocks of the Muda Irrigation Scheme.

Morris, M.B., 1971: Some thoughts on funding your retirement. Northwest Dentistry 50(4): 299 Passim-299 Passim

Neeff, H.; Obermaier, R.; Technau-Ihling, K.; Werner, M.; Kurtz, C.; Imdahl, A.; Hopt, U.T., 2004: Solitary fibrous tumour of the liver: case report and review of the literature. Background. Solitary fibrous tumours (SFTs) of the liver are very rare entities. Although firstly described to be tumours of pleural origin, SFTs have been reported in various organs such as the meninges, orbit, upper respiratory tract, thyroid, p...