geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical demonstration of c-erbB-2 oncoprotein in gastric adenocarcinoma: comparison of cryostat and paraffin wax sections and effect of fixation


, : Immunohistochemical demonstration of c-erbB-2 oncoprotein in gastric adenocarcinoma: comparison of cryostat and paraffin wax sections and effect of fixation. Journal of Clinical Pathology 47(2): 117-121

Aims-To investigate the effects of fixation on the immunohistochemical demonstration of c-erbB-2 oncoprotein using paraffin wax and cryostat sections; to compare c-erbB-2 expression in non-neoplastic and neoplastic gastric tissues. Methods-Adjacent blocks of tumour and non-neoplastic tissue from four gastrectomy specimens were put into a panel of 10 fixatives including acetone, B5, Bouin's fluid, Carnoy's fluid buffered formalin, formol dichromate, zinc formalin, 4% paraformaldehyde, periodate-lysine-paraformaldehyde (PLP) and periodate-lysine-paraformaldehyde-dichromate (PLPD) before embedding in paraffin wax for sectioning. Similar issue blocks were snap frozen and cryostat sections were postfixed in these fixatives, either alone or in combination, before immunostaining. Results-In paraffin wax embedded sections the best fixative was PLP, and in frozen tissues the best results were obtained after fixation of cryostat sections in buffered formalin followed by cold methanol and acetone. Applying these fixatives to samples from a further 16 gastrectomy specimens, strong membrane staining of c-erbB-2 protein was found in the tumour in eight of 16 cases (50%) using paraffin wax sections, and staining was stronger in the better differentiated carcinomas. For frozen tissues, positive membrane staining was found in all gastric adenocarcinomas, but differential staining intensity associated with tumour differentiation could not be detected. Conclusions-These results indicate that fixation and paraffin wax embedding affect the results of immunohistochemical demonstration of c-erbB-2 in gastric cancer. The choice of fixative is critical in the demonstration and evaluation of c-erbB-2 protein expression by immunohistochemistry in gastric carcinomas. Staining results also vary depending on whether frozen or paraffin wax embedded tissues are studied.

US$19.90

PMID: 7907612

DOI: 10.1136/jcp.47.2.117


Other references

Brown, E.M.; Pollak, M.; Hebert, S.C., 1998: The extracellular calcium-sensing receptor: its role in health and disease. The recent cloning of an extracellular calcium (Ca2+o)-sensing receptor (CaR) from parathyroid, kidney and other cell types has clarified the mechanisms through which Ca2+o exerts its direct actions on various cells and tissues. In the parathyroid...

Tanaka, T.; Yoshida, M.; Yokoo, H.; Mizoguchi, K.; Tanaka, M., 1996: ATP-sensitive K+ channel openers block sulpiride-induced dopamine release in the rat striatum. In vivo brain microdialysis was used to investigate the role of ATP-sensitive K+ (KATP) channel openers in dopamine release regulated by dopamine autoreceptors in the rat striatum. Local infusion of two KATP channel openers, nicorandil (10(-5)-10(...

Kang, H.; Park, H.A., 2016: Development of Hypertension Management Mobile Application based on Clinical Practice Guidelines. This study aims to develop and evaluate a mobile application for hypertension management based on Clinical Practice Guidelines. The application was developed according to Web-Roadmap methodology. In planning phase, we defined the tasks and product...

Champeil, P.; Brahms, J., 1974: Conformational properties of some viral DNAs. European Journal of Biochemistry 45(1): 253-259

Zahir, M.; A.M.ttairi, H.R.; Upadhyay, S.Prasad.; Mallick, P.N., 2013: Unilateral Giant Varicocele Mimicking Inguinal Hernia Resulting from Portosystemic Shunt without Evidence of Portal Hypertension: An Unusual Case Report. Isolated giant varicocele has been reported with portal hypertension that results in abnormal communication between portal venous system and testicular vein venous system resulting in retrograde backflow of blood into the testicular venous system...

Moller, E.F.iedrich; Weygand, F.; Wacker, A., 1949: Folic acid as a growth substance for several bacteria; a new folic acid test with Staphylo-coccus-aureus. Teply and Elvehjem's medium, in which the amino acid source was peptone derived from casein by pancreatin, was used as a nutrient medium for lactobacilli cultures. Bacterial inoculations were made in tubes which contained no folic acid or 1.6...

Koenning S.R.; Barker K.R., 1992: Relative damage functions and reproductive potentials of meloidogyne arenaria and meloidogyne hapla on peanut. The reproductive potential and damage functions for Meloidogyne hapla and M. arenaria race 1 on Virginia-type peanut (Arachis hypogaea cv. Florigiant) were determined over 2 years in microplot experiments in North Carolina [USA]. Peanut yield supp...

Winfield, R.J.; Bannister, C.J., 1988: Imazapyr for broad-spectrum weed control in forestry. At various locations during a 2-year period imazapyr at 0.25-1 kg/ha was tested alone and in tank mixtures with atrazine or glyphosate for weed control prior to planting Larix leptolepis, Pinus nigra, Picea sitchensis and Pinus contorta. Treatment...

Duthie, M.S.; Kahn, S.J., 2002: Treatment with a galactosylceramide before Trypanosoma cruzi infection provides protection or induces failure to thrive. Journal of Immunology 168(11): 78-85

Coy, M., 1986: Regional development in the Amazon periphery. Spatial organization, conflicts of interest and regional planning on the 'frontier': the case of Rondonia. Rondonia represents one of the most dynamic frontier zones of the Brazilian Amazon. Over the last few years, the region's development has been characterized by the migration of peasants from the south and southeast of Brazil as a result of mo...