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Immunohistochemical demonstration of carcinogen-DNA adducts in tissues of rats given 2-amino-1-methyl-6-phenylimidazo(4,5-beta)pyridine (PhIP): Detection in paraffin-embedded sections and tissue distribution


, : Immunohistochemical demonstration of carcinogen-DNA adducts in tissues of rats given 2-amino-1-methyl-6-phenylimidazo(4,5-beta)pyridine (PhIP): Detection in paraffin-embedded sections and tissue distribution. Cancer Research 58(19): 4307-4313

A polyclonal antibody against 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP)-DNA adducts was raised for their immunohistochemical demonstration in paraffin-embedded sections. Specificity of this antibody was confirmed by competitive ELISA. Positive signals were immunohistochemically detected in acetone-fixed but not in formalin- or ethanol-fixed sections from F344 rats treated by gavage with a single dose of PhIP at 37.5-300 mg/kg and killed at 1, 2, and 7 days thereafter.

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