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Immunohistochemical demonstration of the plasminogen activator system in human gingival tissues and gingival fibroblasts


, : Immunohistochemical demonstration of the plasminogen activator system in human gingival tissues and gingival fibroblasts. Journal Of Periodontal Research. 33(1): 17-26,.

The relative distribution of urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1) and plasminogen activator inhibitor-2 (PAI-2) was studied in cultured human gingival fibroblasts, healthy gingival tissues and inflamed gingival tissues by immunohistochemistry. In cultured gingival fibroblasts t-PA, u-PA and PAI-1 were expressed in cytoplasm; u-PA and PAI-1 were more intensely stained than t-PA; PAI-2 was not detectable in gingival fibroblasts. Following interleukin 1beta (IL-1beta) stimulation, the intensity of intracellular staining for t-PA was increased and a number of cells staining strongly for PAI-2 were seen; no difference in the intensity of immunostaining level was noted for the expression of u-PA and PAI-1 between IL-1beta stimulated cells and unstimulated cells. In healthy gingival tissues, u-PA and PAI-1 displayed a wide distribution throughout all the connective tissue and epithelium; t-PA localized mainly in the connective tissue while PAI-2 showed little association with the connective tissue but did faintly stain in the epithelial layer. In inflamed gingival tissues, staining for t-PA was significantly increased in the extracellular matrix of the connective tissue, whereas staining for u-PA, PAI-1 and PAI-2 was found to be slightly increased, but no significant difference was noted for staining when compared with the healthy gingival tissues. A granular distribution of t-PA, u-PA, PAI-1 and PAI-2 was noted around areas of inflammatory cell infiltration. These immunohistochemical findings indicate that the plasminogen activator system produced by fibroblasts may be influenced by the presence of the inflammatory mediator IL-1beta. In addition, the significant increase of t-PA in inflamed connective tissue and the wide expression of these components around inflamed cells may contribute to connective tissue degradation and may relate to the migration and localization of monocytes/macrophages in inflamed tissue.

US$19.90

PMID: 9524317

DOI: 10.1111/j.1600-0765.1998.tb02287.x


Other references

V.W.K.Lee, 1984: Control of inhibin secretion in polytocous animal species. Journal of Steroid Biochemistry 20(6-part-P2): 1

Rivier, C.; Vale, W., 1989: In the rat, interleukin-1 alpha acts at the level of the brain and the gonads to interfere with gonadotropin and sex steroid secretion. These studies were designed to investigate a possible effect of the monokine interleukin-1 alpha (Il-1 alpha) on gonadotropins and/or sex steroid secretion. Three putative sites of action were considered: the central nervous system, the pituitary,...

Lacassagne, A., 1939: Sex hormones and cancer formation. The development of mammary and other tumors in mice as influenced by estrogenic hormones is reviewed. It is concluded from the work on the subject that if estrogenic substances do possess carcinogenic properties, long periods of action and relativ...

Miyashita, N.; Matsumoto, A.; Kubota, Y.; Nakajima, M.; Niki, Y.; Matsushima, T., 1996: Continuous isolation and characterization of Chlamydia pneumoniae from a patient with diffuse panbronchiolitis. We succeeded in isolating Chlamydia pneumoniae organisms continuously from a 70-year-old man who had received chemotherapeutic treatment with low dosages of erythromycin for five years to improve diffuse panbronchiolitis (DPB). He had two episodes...

McGehee, A.M.; Strijbis, K.; Guillen, E.; Eng, T.; Kirak, O.; Ploegh, H.L., 2011: Ubiquitin-dependent control of class II MHC localization is dispensable for antigen presentation and antibody production. Controlled localization of class II MHC molecules is essential for proper class II MHC-restricted antigen presentation and the subsequent initiation of an adaptive immune response. Ubiquitination of class II MHC molecules on cytosolic lysine (K225...

Jafarian, A.Akbar.; Imani, F.; Salehi, R.; Najd Mazaher, F.; Moini, F., 2016: Simple Arm Tourniquet as an Adjunct to Double-Cuff Tourniquet in Intravenous Regional Anesthesia. Intravenous Regional Anesthesia (IVRA) is a well-known technique for producing analgesia during surgical procedures in the extremities. However, the rapid onset of pain following the deflation of a double-cuff tourniquet during IVRA is a serious d...

Mullen, J.C.; Bentley, M.J.; Scherr, K.D.; Chorney, S.G.; Burton, N.I.; Tymchak, W.J.; Koshal, A.; Modry, D.L., 2002: Troponin T and I are not reliable markers of cardiac transplant rejection. Heart transplant recipients undergo a number of invasive endomyocardial biopsies to screen for rejection. Serum assays of troponin T and/or I may provide a less invasive alternative. The purpose of this study was to evaluate troponin T and I as ma...

Komp D.; George S.; Land V.; Fernbach D.; Humphrey G.B.; Lowman J., 1974: Response and toxicity in childhood leukemia when cyclo phosphamide and l asparaginase are added to vincristine prednisone induction. Proceedings of the American Association for Cancer Research 15: 169

Takahashi, S.; Nagano, Y.; Nagano, N.; Fujita, K.; Taguchi, F.; Okuwaki, Y., 1993: Opsonisation of group B streptococci and restriction endonuclease digestion patterns of their chromosomal DNA. Isolates of group B streptococci (GBS) from neonates with early-onset septicaemia are associated with particular restriction endonuclease digestion patterns (RDP types Ia-3 and III-3) of chromosomal DNA. Opsonophagocytosis of serotype Ia and serot...

Andjelkovicj M., 1986: Geodynamic processes and stratigraphic-paleogeographic development of Oligocene and Miocene freshwater basins in central and western Serbia. Geoloshki Anali Balkanskoga Poluostrva 50: 1-74