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Immunohistochemical detection and northern blot analysis of estrogen receptor in osteoblastic cells


, : Immunohistochemical detection and northern blot analysis of estrogen receptor in osteoblastic cells. Journal of Bone and Mineral Research 8(9): 1103-1109

Expression of estrogen receptor (ER) was studied in MC3T3-E1 cells (mouse osteoblastic cell line), HOS TE85 cells (human osteosarcoma cell line), and primary osteoblastic cells derived from mouse calvaria with immunohistochemical techniques. The staining of ER was readily detectable in MC3T3-E1 cells, HOS TE85 cells, and primary osteoblastic cells by using a monoclonal anti-ER antibody that recognizes the DNA binding domain of ER. The immunoreactivity was distributed in the cytoplasm as well as in the nuclei. 17 beta-Estradiol (10(-8) M) did not alter this staining pattern. The expression of ER was confirmed by Northern blot analysis using rat ER cDNA probe, which revealed a 6.5 kb band in MC3T3-E1 cells and a 6.2 kb band in HOS TE85 cells. The mRNA level of ER was not altered by 17 beta-estradiol (10(-8) M). The immunohistochemical studies showed that ER was not detectable in all cells but in a small population of each cell type. This study is the first report to demonstrate the presence of ER immunohistochemically, and our results suggest the heterogeneity of ER expression among osteoblastic cells.

US$19.90

PMID: 8237480

DOI: 10.1002/jbmr.5650080911


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Dellacà, R.L.; Zannin, E.; Ventura, M.L.; Sancini, G.; Pedotti, A.; Tagliabue, P.; Miserocchi, G., 2013: Assessment of dynamic mechanical properties of the respiratory system during high-frequency oscillatory ventilation*. 1) To investigate the possibility of estimating respiratory system impedance (Zrs, forced oscillation technique) by using high-amplitude pressure oscillations delivered during high-frequency oscillatory ventilation; 2) to characterize the relation...

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Barasolo E.; Bodo G.; Bossola M.; Ventura M., 1982: Deferento vesiculography in the detection of cancer of the prostate. Journal d'Urologie 88(3): 188-189

Hanson, M.J., 1994: How we treat the elderly. Hastings Center Report 24(5): 4-6

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Rosenberg, H., 1983: Relapsed vs. nonrelapsed alcohol abusers coping skills life events and social support. Based on Marlatt's cognitive-behavioral relapse model and the results of several suggestive research studies, data were gathered on coping skills, life events and social support to investigate the differences between relapsed and non-relapsed...

Quimby, E.D., 1984: Contractors with consultant's hats. Trustee 37(2): 26-26