geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical detection of RAS, JUN, FOS, and p53 oncoprotein expression in human colorectal adenomas and carcinomas


, : Immunohistochemical detection of RAS, JUN, FOS, and p53 oncoprotein expression in human colorectal adenomas and carcinomas. Laboratory Investigation; A Journal of Technical Methods and Pathology 69(6): 674-681

BACKGROUND: Recent evidence suggests that progressive stages of colorectal tumorigenesis can be defined by a sequence of genetic events characterized by deletion and expression of certain genes and appearance of several oncoproteins. Although the significance of these events is not entirely clear, oncoprotein expression may be directly involved in the tumorigenic mechanism. EXPERIMENTAL DESIGN: We examined the expression of two nuclear oncoproteins, JUN and FOS (Abbreviations used in this paper are lower case letters for oncogenes and upper case letters for oncoproteins), that have not been previously associated with development of colorectal cancer. This study involved detecting p39 c-JUN and p55 c-FOS, as well as two oncoproteins previously known to be expressed during colorectal tumorigenesis, p21 RAS and the tumor suppressor protein, p53. Expression was detected with immunohistochemical methods on formalin-fixed, paraffin-embedded sections of normal human colons, tubular adenomas, tubulovillous adenomas, and adenocarcinomas. Either single oncoprotein expression or coexpression of four selected pairs; RASI JUN, RAS/FOS, RAS/p53, or JUN/FOS were evaluated. RESULTS: We found that the expression of all four single oncoproteins and oncoprotein pairs were detected in very few normal colon specimens or tubular adenomas. However, all four oncoproteins and the four oncoprotein pairs were expressed significantly more often in adenocarcinomas. Oncogene expression in tubulovillous adenomas varied with lesion size. The number of lesions expressing any of the four oncoproteins or pairs was not significantly different than normal colon in small ( lt 1 cm diameter) tubulovillous adenomas. Significantly more large lesions ( gt 1 cm diameter) expressed the single oncoproteins RAS, JUN, and FOS than normal colon. Of the four oncoprotein pairs, only RAS/JUN was significantly coexpressed in large tubulovillous adenomas. CONCLUSIONS: We conclude that independent expression of RAS, JUN, and FOS occurred significantly more often in large tubulovillous adenomas and adenocarcinomas while p53 expression occurred primarily in adenocarcinomas. Also, although all four oncoprotein pairs were expressed significantly more often in adenocarcinomas, only RAS/JUN was significantly coexpressed in the large tubulovillous adenomas. These results are comparable with the observed sequential activation of Ki-ras and p53 described by others and suggests that the expression of the nuclear oncogenes, JUN and/or FOS, are also important events in colorectal tumorigenesis.

US$29.90

PMID: 8264230


Other references

Tanaka, S.; Takanashi, S., 1975: Studies on the tolerance of pear black spot fungus (Alternaria kikuchiana Tanaka) against an antibiotic, polyoxin. Str. A-2 of A. kikuchiana showed high tolerance of polyoxin and strong pathogenicity to the pear cv. Nijisseiki. Another 3 pathogenic strs. of the conidial type showed equal pathogenicity but high sensitivity to polyoxin, while 3 mycelial type str...

Morris, O.N., 1966: Incorporation of radioactive uridine into the RNA of the Lipidopteran Barathra brassicae. Journal of Invertebrate Pathology 8(2): 259-261

Bardin, T.; Caroit, M., 1990: Scapulohumeral periarthritis. Etiology, physiopathology, diagnosis and principles of treatment. La Revue du Praticien 40(10): 959-964

Parli, C.J.; Mannering, G.J., 1970: Induction of drug metabolism. IV. Relative abilities of polycyclic hydrocarbons to increase levels of microsomal 3-methyl-4-methylaminoazobenzene N-demethylase activity and cytochrome P1-450. Molecular Pharmacology 6(2): 178-183

Drahota, A.; Aarons, G.A.; Stahmer, A.C., 2013: Developing the Autism Model of Implementation for autism spectrum disorder community providers: study protocol. Currently, 1 out of 88 children are diagnosed with an autism spectrum disorder (ASD), and the estimated cost for treatment services is $126 billion annually. Typically, ASD community providers (ASD-CPs) provide services to children with any severi...

Hughes, A.F.W., 1949: The heart output of the chick embryo. Cinemicrographs of the exposed hearts of chick embryos (2.5-17 days' incubation) were taken with a camera working at 16 frames per sec. Paper enlargements or projection drawings of the outline of the ventricle were made from single frames fro...

Vazquez-Tello, A.; Castán, P.; Moreno, R.; Smith, J.M.; Berenguer, Jé.; Cedergren, R., 2002: Efficient trans-cleavage by the Schistosoma mansoni SMalpha1 hammerhead ribozyme in the extreme thermophile Thermus thermophilus. The catalytic hammerhead structure has been found in association with repetitive DNA from several animals, including salamanders, crickets and schistosomes, and functions to process in cis the long multimer transcripts into monomer RNA in vivo. Th...

Hayashi-Ishimaru, Y.; Ehara, M.; Inagaki, Y.; Ohama, T., 1997: A deviant mitochondrial genetic code in prymnesiophytes (yellow-algae): UGA codon for tryptophan. The sequence of a representative mitochondrial gene COXI, encoding cytochrome c oxidase subunit I, was determined in five species that cover all the orders of the Prymnesiophyta with the exception of the Pavlovales. Through this analysis, we notic...

D.D.E.M.A.; Kuipers.E.; Noach.E.L., 1956: The influence of phenacetin on adrenal activity. In order to investigate whether the typical action of antipyretics-analgetics is mediated by the adrenal gland, phenacetin was chosen as a relatively non-toxic drug, which is easily voluntarily ingested by rats. Doses which were much lower than th...

Barta, L.; Rosta, J., 1960: On juvenile obesity. Gyermekgyogyaszat 11: 131-141