geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical detection of aberrant p53 expression in hepatocellular carcinoma: Correlation with cell proliferative activity indices, including mitotic index and MIB-1 immunostaining


, : Immunohistochemical detection of aberrant p53 expression in hepatocellular carcinoma: Correlation with cell proliferative activity indices, including mitotic index and MIB-1 immunostaining. Human Pathology 26(3): 326-333

We analyzed the p53 expression immunohistochemically in 50 specimens of hepatocellular carcinoma (HCC) using two monoclonal antibodies (DO7 and PAb1801) and one polyclonal antibody (CM1), which recognize both wild and mutant type p53 proteins and can be used for paraffin-embedded sections. Fifteen of the 50 HCC specimens (30%) showed p53 expression localized at tumor nuclei, and this expression was significantly more frequent in HCCs with histologically lower differentiation. Except for serum titers of alpha-fetoprotein, the p53 expression had no statistically significant correlation with clinicopathological parameters, including hepatitis virus infection, tumor size, and background liver diseases. Conversely, the cell proliferative activities of tumor cells as assessed by mitotic index and immunostaining for MIB-1 were well correlated with the grade of histological differentiation. Moreover, MIB-1 immunostaining was shown to be useful in distinguishing well differentiated HCC from hepatocytes in chronic liver diseases. It also was shown that p53 expression was strongly associated with cell proliferative activity. Our results indicate that p53 expression takes place in the late stage of tumor progression and is related to the high malignant potential of HCCs.

US$19.90

PMID: 7890286

DOI: 10.1016/0046-8177(95)90066-7


Other references

Kjeldsen, S.E.; Messerli, F.H.; Chiang, C-En.; Meredith, P.A.; Liu, L., 2013: Are fixed-dose combination antihypertensives suitable as first-line therapy?. To contemplate how initial antihypertensive therapy with fixed-dose combinations (FDC) might be incorporated into clinical practice, based on a compilation of evidence comparing FDCs with monotherapy and loose-dose combinations in varying patient...

Murray, B.L.; Barnard, K.E., 1966: The nursing specialist in mental retardation. Nursing Clinics of North America 1(4): 631-640

Burgass, R.W.; Powell, A.A., 1984: Evidence for repair processes in the invigoration of seeds by hydration. Brussels sprout seeds with low vigour caused by aging showed a large improvement in seed quality following a 2-h soak in water. This was reflected in an increased rate of germination, higher emergence in soil, and the retention of high germination...

Poetker, D.M.; Smith, T.L., 2016: Medicolegal Implications of Common Rhinologic Medications. As otolaryngologists, we prescribe many medications to our patients. The objective of this article is to review the potential side effects and medicolegal risks of the common medications used to treat chronic rhinosinusitis. The authors evaluate s...

Aas, M.; Jonáš, A.; Kiraz, A.; Brzobohatý, O.; Ježek, J.; Pilát, Zěk.; Zemánek, P., 2013: Spectral tuning of lasing emission from optofluidic droplet microlasers using optical stretching. We introduce tunable optofluidic microlasers based on active optical resonant cavities formed by optically stretched, dye-doped emulsion droplets confined in a dual-beam optical trap. To achieve tunable dye lasing, optically pumped droplets of oil...

Brekke, M., 2002: A holistic health care 'bill of rights'. Beginnings 21(5): 9-9

Shmaraev, G.E., 1971: Results of a study on a collection of table maize for resistance to pests. Among almost 1000 forms tested, certain types resistant to Oscinella frit were found among the popcorns and sweet corns whereas most of the other types were susceptible. In all groups, inbreds were more susceptible than varieties and hybrids. Reac...

Bell, H., 1971: Cardiac manifestations of viral hepatitis. JAMA 218(3): 387-391

Raut, J.G., 1995: Management of seedborne fungi of pearl millet in storage. Indian Journal of Mycology & Plant Pathology 25(1-2): 39

Schram, A.W.; De-Groot, P.G.; Hamers, M.N.; Brouwer-Kelder, B.; Donker-Koopman, W.E.; Tager, J.M., 1978: Further characterization of 2 forms of n acetyl alpha galactosaminidase from human liver. Two forms of N-acetyl-.alpha.-galactosaminidase (EC 3.2.1.22) were isolated from human liver using Sepharose 4B-concanavalin A chromatography, followed by CM-cellulose chromatography. Both forms hydrolyze not only p-nitrophenyl-N-acetyl-.alpha.-ga...