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Immunohistochemical detection of activin A in osteoclasts


, : Immunohistochemical detection of activin A in osteoclasts. Gerontology 42 Suppl 1: 20-24

Production of a member of transforming growth factor-beta (TGF-beta) superfamily, activin A, was examined in the bone tissue by using reverse transcriptase polymerase reaction. As a result, specific bands were detected showing the presence of activin A mRNA in the bone tissues. In order to localize the production site of activin A in the bone tissues, we tried to immunolocalize activin A in fetal mouse calvaria cultured in a medium containing fetal calf serum, 1-alpha-25(OH)-2 vitamin D-3 and parathyroid hormone. In these cultured calvaria, bone tissues including bone-resorbing osteoclasts in vitro were observed. Positive staining demonstrating the presence of activin A resided inside of the multinucleated cells in the bone-resorbing lacunae, suggesting the production of activin A in osteoclasts. Activin A was also localized immunohistochemically in the osteoclast-like multinucleated cells developed in vitro. These results suggest that osteoclasts produce activin in the bone tissues and that activin may play some roles by autocrine and/or paracrine manner in bone metabolisms.

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PMID: 8964519


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