+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical detection of alkaline phosphatase in formalin-fixed and paraffin-embedded rat liver

, : Immunohistochemical detection of alkaline phosphatase in formalin-fixed and paraffin-embedded rat liver. Okajimas Folia Anatomica Japonica 70(4): 151-155

Alkaline phosphatase (ALP) in rat liver was detected by means of immunohistochemical techniques in ordinary histologic specimens which were fixed with formalin and embedded in paraffin. The specimens were incubated in anti-ALP antibody at room temperature for a longer time, 3 hr, than ordinary incubation time. Antigen detection sensitivity and contrast of reaction product of the immunogold sliver staining were superior to those of the peroxidase-labeled antibody method. In rat livers which were partially hepatectomized 24 hr before, the more abundant enzyme localized around enlarged bile canalicular spaces.


PMID: 8272319

Other references

Martens, P., 1936: Megasporange et relief cortical chez Pinakodendron ohmanni. Details of the structure of fruiting specimens of P. ohmanni* are descr.

Forsyth F.R.; Eaves C.A., 1975: Ripening of apples in controlled atmosphere storage low or high ethylene levels and medium or high humidity levels. Ulrich, R (Ed ) Colloques Internationaux Du Centre National De La Recherche Scientifique, No 238 Facteurs Et Regulation De La Maturation Des Fruits (International Colloquia Of The National Center For Scientific Research, No 238 Factors And Regulation Of The Maturation Of Fruits Paris, France, July 1-5, 1974 368p Illus Editions Cnrs: Paris, France Isbn 2-222-01788-2 67-72

Montagnino, G.; Colturi, C.; Tarantino, A.; Masa, A.; Banfi, G.; Aroldi, A.; ViganĂ³, E.; Cesana, B.; Ponticelli, C., 1991: The impact of azathioprine and cyclosporine on long-term function in kidney transplantation. To assess the impact of cyclosporine on long-term kidney function in transplant patients, we retrospectively analyzed 273 patients on azathioprine and 308 on CsA with graft functioning at 1 year. To balance the length of follow-ups, the observatio...

Rock, M.L.; Fox, B.H., 1949: Two aspects of the Pulfrich phenomenon. American Journal of Psychology 62(2): 279-284

Suryawanshi, A.; Veiga-Parga, T.; Rajasagi, N.K.; Reddy, P.Babu.Jagdeesh.; Sehrawat, S.; Sharma, S.; Rouse, B.T., 2011: Role of IL-17 and Th17 cells in herpes simplex virus-induced corneal immunopathology. HSV-1 infection of the cornea leads to a blinding immunoinflammatory lesion of the eye termed stromal keratitis (SK). Recently, IL-17–producing CD4<sup>+</sup> T cells (Th17 cells) were shown to play a prominent role in many auto...

Nakazato, S.; Ikenishi, K., 1989: Monoclonal antibody production against a subcellular fraction of vegetal pole cytoplasm containing the germ plasm of Xenopus 2-cell eggs. In attempts to understand the molecular nature of substances localized in the germ plasm (GP), monoclonal antibodies against a subcellular fraction of vegetal pole cytoplasm containing the GP of Xenopus 2-cell eggs were produced. The monoclonal an...

Lopez A.M.; Torres C.L.; Wang H.; Goldberg R.N., 1990: A reappraisal of gentamicin dosage guidelines for neonates the value of postconceptional age pca dosing. Pediatric Research 27(4 PART 2): 62A

Stores, G., 1992: A clinical approach to poorly controlled seizures in children. Uncontrolled seizures are a serious problem and a source of much anxiety to physicians, parents and others concerned with the care of children with epilepsy. A systematic approach to the causes of poor seizure control, considering basic diagnostic...

Maneeboon, T.; Vanichsriratana, W.; Pomchaitaward, C.; Kitpreechavanich, V., 2010: Optimization of lactic acid production by pellet-form Rhizopus oryzae in 3-L airlift bioreactor using response surface methodology. The influence of two key environmental factors, pH and oxygen transfer coefficient (k(L)a), was evaluated on the lactic acid production as the main answer and, on the size of cell pellets of the fungal strain Rhizopus oryzae KPS106, as second depe...

Maalikjy Akkawi, N.; Borroni, B.; Magoni, M.; Vignolo, L.A.; Padovani, A., 2002: Lemierre's syndrome complicated by carotid thrombosis. Lemierre's syndrome, also known as postanginal sepsis, is a rare condition that presents as an increasing sore throat due to acute pharyngitis or tonsillitis and progresses to sepsis, due to suppurative thrombophlebitis of the internal jugula...