geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical detection of an enamel protein-related epitope in rat bone at an early stage of osteogenesis


, : Immunohistochemical detection of an enamel protein-related epitope in rat bone at an early stage of osteogenesis. Histochemistry. 99(5): 355-362

Monoclonal antibody M1315 was produced against hamster tooth germ homogenate by in vitro immunization. It was found that M1315 reacted with enamel matrix, ameloblasts, and bone matrix at an early stage of osteogenesis. Decalcified tissues of rat femurs and mandibles were examined with M1315 using indirect immunofluorescence. In endochondral ossification of femurs, immunoreactivity was found in bone extracellular matrix (ECM) deposited on the surface of the cartilage core of primary spongiosa, but not in the cartilage core itself. In intramembranous ossification of 0-day-old rat mandibles, intense immunofluorescence was detected in bone ECM and a few young osteocytes, but not in osteoblasts. Immunoreactivity in bone ECM of 2-day-old rats decreased and almost disappeared from bone ECM of 4-day-old rats. Although in nondecalcified sections of 0-day-old rats, negligible immunofluorescence was detected in bone ECM which showed positive staining in decalcified tissues, the immunostaining appeared after decalcification using ethylenediaminetetraacetic acid (EDTA). These results indicate that a substance(s), which had a common epitope with an enamel-derived protein(s), existed in immature bone ECM of both endochondral and intramembranous ossification, and that it might be masked by bone mineral. Monoclonal antibody M1315 is a useful tool to investigate the time- and position-specific changes in osteogenesis and amelogenesis.

US$19.90

PMID: 7687593

DOI: 10.1007/bf00717047


Other references

Tan, K.R.; Cullen, K.A.; Koumans, E.H.; Arguin, P.M., 2016: Inadequate Diagnosis and Treatment of Malaria Among Travelers Returning from Africa During the Ebola Epidemic--United States, 2014-2015. Among 1,683 persons in the United States who developed malaria following international travel during 2012, more than half acquired disease in one of 16 countries in West Africa. Since March 2014, West Africa has experienced the world's larges...

Keleti, G.; Fekete, F., 1988: Evaluation of a percutaneous transhepatic prosthesis of the biliary tract based on 58 cases. Orvosi Hetilap 129(15): 775-778

Saleem, M.A.; Shakoori, A.R., 1986: Starvation and refeeding in Tribolium castaneum (Herbst). I. Effect on some biochemical components of sixth instar larvae. In connection with studies on the possible effects of starvation on the efficacy of insecticides against the tenebrionid Tribolium castaneum in stored grain, 5 days of starvation led to a significant increase in the amylase activity of 6th-instar...

Tian, J.; Cai, T.; Haas, M.; Li, D.; Xie, Z., 2003: Sodium pump as a signal transducer Interaction with Src. Biophysical Journal 84(2 Part 2): 267a, February

Kavun, S.; Fedorova, T.; Akin'-Shina, G.; Tarasova, Z.; Khodzhayeva, I., 1973: Reaction of p-nitrosodiphenylamine with isoprene rubber. A study was made of the reaction of p-nitrosodiphenylamine (PNDPA) with isoprene rubber and it was established that it takes place through nitroso-groups and results in the formation of at least two N-phenyl-p-phenylenediamine derivatives, of whic...

Friend, JA., 1987: Local decline, extinction and recovery: relevance to mammal populations in vegetation remnants. Unknown

Ferri, S.; Pallini, V.; Pellerano-Dòmini, I., 1966: Chromatographic isolation of ricinin from castor beans. Il Farmaco; Edizione Scientifica 21(7): 511-516

Ruppel E.G., 1985: Effect of tillage and herbicides on fusarium stalk rot of corn. Phytopathology: 1296

Schapiro, Va, 1971: On the nature of ontogenetic interrelationships among several groups of parasitic insects and their hosts. International Congress of Entomology 13th Moscow 1968 Trudy: 182-184

Lim, J.; Yeap, S.Pin.; Leow, C.Hoe.; Toh, P.Yi.; Low, S.Chun., 2015: Magnetophoresis of iron oxide nanoparticles at low field gradient: the role of shape anisotropy. Magnetophoresis of iron oxide magnetic nanoparticle (IOMNP) under low magnetic field gradient (<100 T/m) is significantly enhanced by particle shape anisotropy. This unique feature of magnetophoresis is influenced by the particle concentration...