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Immunohistochemical detection of cells labeled with bromodeoxyuridine after neural transplantation


, : Immunohistochemical detection of cells labeled with bromodeoxyuridine after neural transplantation. Molecular & Chemical Neuropathology. 25(2-3): 235-245

Pregnant rats were treated with 5-bromo-2'-deoxyuridine (BrdU) from embryonic d 12 (ED12) to ED14. BrdU administration allowed timed labeling of dividing embryonic cells in utero, since the drug is incorporated into the DNA in place of thymidine during the S-phase of the cell cycle. ED14 rat cerebral cortex or placenta was grafted into the brain of adult rats. Anti-bromodeoxyuridine immunohistochemistry was used for identifying labeled transplanted cells after different survival periods in paraffin-embedded sections. BrdU labeled cells were observed in both intraventricular and intraparenchymal cortical grafts, even after a 3-mo survival. Although the percentage of positive cells decreased in comparison with ED14 cortex, the level of BrdU (i.e., the intensity of anti-BrdU immunohistochemistry) in labeled nuclei was probably the same. BrdU pretreatment of ED14 cells prior to grafting did not affect the proliferative ability of the grafted tissue. In ED14 placental grafts, all trophoblastic cells were labeled distinctly. This precise labeling technique enabled an examination of individual migrating trophoblastic cells. However, migration of these cells into the host brain was very limited.

US$19.90

PMID: 8534324

DOI: 10.1007/bf02960916


Other references

Hashiguchi, T.; Maeda, Y.; Kashima, M.; Tanoue, Y.; Yamaji, M., 1982: The effectiveness of on-farm progeny testing on the meat production of Japanese Black cattle. Data on 3431 steers were analysed. Significant sire effects were found for all traits examined: these were initial body weight, age at the beginning of fattening, initial weight for age, fattening duration, final body weight, daily gain during the...

Kusnetzow, I.D., 1908: Un exemplaire de Barbus brachycephalus Kissl trouve dans la Volga en amount de Tsaritsyn. Annales du Musee Zoologique St Peterburg, 13 (xlv)

Plan Lukas, 2005: Factors controlling carbonate dissolution rates quantified in a field test in the Austrian Alps. Carbonate dissolution rates were investigated by measuring the mass difference of carbonate tablets exposed to natural dissolution for 1 year. 70 tablets were distributed over 13 test sites on the north slope of the Hochschwab Massif in the Austri...

Coram, R.J.; Stillwagon, S.J.; Guggilam, A.; Jenkins, M.W.; Swanson, M.S.; Ladd, A.N., 2016: Muscleblind-like 1 is required for normal heart valve development in vivo. Development of the valves and septa of the heart depends on the formation and remodeling of the endocardial cushions in the atrioventricular canal and outflow tract. These cushions are populated by mesenchyme produced from the endocardium by epith...

Norton-Griffiths, M., 1969: The organisation control and development of parental feeding in the oystercatcher (Haematopus ostralegus). Behaviour, 34: 55-114

Meade, R.H., 1977: Treatment of chronic mucocutaneous candidiasis. The case of a 4-yr-old woman who had been unsuccessfully treated for 20 yr with various agents for Candida albicans infection (amphotericin B, nystatin, transfer factor, 5-fluorocytosine) with only temporary regression, but was successfully treate...

Royles, D., 2014: Put values into practice. Health Service Journal Suppl: 14-15

Elms, T.N.; Buschang, P.H.; Alexander, R.G., 1996: Long-term stability of Class II, Division 1, nonextraction cervical face-bow therapy: I. Model analysis. The long-term stability of Class II, Division 1 nonextraction therapy remains poorly described. This study evaluates the face-bow therapy, in conjunction with full-fixed appliance therapy, of 42 patients (34 females and 8 males) who were treated b...

Miller, E.J.; Chibnall, A.C., 1932: The proteins of grasses: Preliminary communication. Biochemical Journal 26(2): 392-402

Kurotaki, M.; Takano, K., 1971: Myoepithelial cells showing exceptional fractures in the dermal gland of Rana japonica. Kaibogaku Zasshi. Journal of Anatomy 46(4): 224-236