+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical detection of nucleolar protein p120 in paraffin-embedded tissues

, : Immunohistochemical detection of nucleolar protein p120 in paraffin-embedded tissues. Histochemical Journal. 29(9): 661-668,.

The monoclonal antibody FB-2 recognizes the antigen p120-kDa protein (p120), associated with the nucleolar matrix. p120 has originally been reported as expressed and detectable in malignant and non-neoplastic proliferating cells, but not in most normal resting tissues and benign tumours. In the present study, a reliable immunostaining method was used to detect p120 on formalin-fixed, paraffin wax-embedded tissue, testing it on 148 samples from different neoplastic and non-neoplastic tissues from different organs (breast, colon, lung, prostate, bladder, lymph nodes, skin, tongue and liver). The immunostaining was performed after the application of a specific antigen-unmasking protocol based on six consecutive cycles of microwave oven heating. Under these retrieval conditions, p120 antigen was dearly detectable, not only in hyperplastic and malignant cells, but also in stromal and normal non-proliferating cells of all the tissues evaluated. Our results show that the nucleolar protein p120 can be detected by routine immunohistochemistry in formalin-fixed, paraffin-embedded tissue and is expressed in all nucleated cells under any biological condition.


PMID: 9413739

DOI: 10.1023/a:1026400731485

Other references

Peter, C.A.; Bowyer, K.; Jones, R.H., 1983: Radionuclide analysis of right and left ventricular response to exercise in patients with atrial and ventricular septal defects. In patients with ventricular or atrial septal defect, the ventricle which is chronically volume overloaded might not appropriately respond to increased demand for an augmentation in output and thereby might limit total cardiac function. In this st...

Hafez,A.M.A.; Abdelhady,H.M.; Sharaf,M.S.; El-Tayeb,T.S., 2008: Improvement of pullulan production by Aureobasidium pullulans under high cell density inoculation two-stage batch fermentation system. Pullulan production by <i>Aureobasidium pullulans</i> ATCC 42023 was studied in bioreactor as a high cell density inoculation (HCDI) in two-stage batch culture. Pullulan formation was examined at different agitation rates (100-900 rpm)...

Hallman, F.A.; Delamater, J.N., 1953: The demonstration of amylolytic activity in cultures of Endamoeba histolytica. A bacteriological method developed by Andrewes was adapted to demonstrate starch hydrolysis by living cultures of E. histolytica. Sterile "peni-cylinders" were set into sterile agar plates (Bacto Blood Agar Base) containing 0.3% soluble...

Deli, G.; Jin, C-Hao.; Mu, R.; Yang, S.; Liang, Y.; Chen, D.; Makuuchi, M., 2005: Immunohistochemical assessment of angiogenesis in hepatocellular carcinoma and surrounding cirrhotic liver tissues. To investigate whether vascular endothelial growth factor (VEGF) was over-expressed in hepatocellular carcinoma (HCC) or in surrounding cirrhotic liver tissues. Immunohistochemistry was performed to investigate the expression of VEGF proteins in H...

Gassies., 1867: Of Bulimus acutus by Gassies, Malacol. de l'Aquit p. 19. of Bulimus acutus by Gassies, Malacol. de l'Aquit p. 19

Ruchkin G.V.; Ivakin A.N.; Shnayder M.S.; Shnayder A.A.; Rodionov S.M.; Levshuk A.Y.; Orlovskiy V.V.; Larioshkin A.K.; Akimov V.M., 1986: Geologic structure and genetic characteristics of a tin-tungsten deposit of the stockwork type in Primorye. Tikhookeanskaya Geologiya = Pacific Geology 1986(2): 68-75

Triaskov, A.A., 1977: Microsurgical operations in ophthalmology. Voenno-Meditsinskii Zhurnal: 19-23

Von Bodungen, U.; Lechner, F.; Pfister, H.; Vogt, H.R.; Cheevers, W.P.; Bertoni, G.; Jungi, T.W.; Peterhans, E., 1998: Immunohistology of the early course of lentivirus-induced arthritis. Caprine arthritis encephalitis (CAE) is a lentiviral infection of goats characterized by mononuclear cell infiltration of various tissues, most prominently the joints, mammary glands and, in young animals, the brain. We have investigated the early...

Houli, J., 1954: Introduction to the history of rheumatology. Arquivos Brasileiros de Medicina 44(7-8): 267-278

Schoene, G.; Yeager, T., 2005: Micropropagation of sweet viburnum (Viburnum odoratissimum). A micropropagation protocol for shoot culture of sweet viburnum (Viburnum odoratissimum) is described. Nodal explants, initially established on MS medium, were transferred to WPM supplemented with combinations of BA and GA3. Maximum shoot multipli...