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Immunohistochemical detection of prostaglandin I-2 synthase in various calcified tissue-forming cells in rat

, : Immunohistochemical detection of prostaglandin I-2 synthase in various calcified tissue-forming cells in rat. Archives Of Oral Biology. 38(1): 31-36

Localization of prostaglandin (PG) I-2 synthase immunoreactivity was examined in demineralized sections of rat pulpal, periodontal and skeletal tissues using isn-1, a monoclonal antibody raised against the enzyme. Various calcified tissue-forming cells, i.e. odontoblasts, osteoblasts, osteocytes, cementoblasts, cementocytes and chondrocytes, were similarly immunoreactive for PGI-2 synthase, suggesting that they are capable of producing PGI-2. In odontoblasts and chondryocytes, the reactivity increased gradually with maturation. Weak immunoreactivity was also observed in endothelial cells and fibroblast-like cells in pulpal and periodontal tissues. Howver, no reactivity was seen in ameloblasts. These results suggest the possible involvement of PGI-2 in the regulation of the metabolism of various calcified tissues. Monoclonal antibodies such as isn-1 may become useful markers of the maturation of calcified tissue-forming cells of mesenchymal origin.


PMID: 8442718

DOI: 10.1016/0003-9969(93)90151-b

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