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Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens

, : Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens. Journal of Pathology 184(1): 77-82

The nucleolar organizer regions (NORs) of human chromosomes can be identified in interphase and mitotic cells by localization of some intrinsic components such as the associated enzyme RNA polymerase I. A new sensitive staining method for NORs is described using a specific antibody to the ribosomal transcription factor UBF. By indirect immunofluorescence and enzyme-labelling methods, NORs stained in benign and malignant cells from a variety of tissues with monospecific anti-UBF serum showed significant morphological differences which correlated well with histopathological evaluation. The number of NORs per cell in malignant preparations increased significantly. Furthermore, the staining of a NOR protein component such as UBF appears to be as sensitive as the silver-staining technique (AgNOR) and might be a better alternative for detecting ribosomal activity in malignant tissues.


PMID: 9582531

DOI: 10.1002/(SICI)1096-9896(199801)184:1<77::AID-PATH958>3.0.CO;2-X

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