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Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pre-treatment

, : Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pre-treatment. Journal of Histochemistry and Cytochemistry 42(8): 1169-1175

We describe the immunohistochemical detection of the human androgen receptor (AR) in routinely processed, paraffin-embedded tissue with the monoclonal antibody (MAb) F39.4. Deparaffinized sections were heated in a microwave oven for antigen retrieval. A panel of human male- and female-derived tissues was investigated. We observed a nuclear staining pattern consistent with previous results on frozen sections. Moreover, we studied the possibility of detecting AR in prolonged formalin-fixed tissue and in paraffin-embedded archival material. After prolonged fixation times or long-term storage of paraffin-embedded tissue, the staining intensity for the AR did not deteriorate. Blocking experiments with the specific synthetic peptides demonstrated the specificity of this technique. We conclude that this method is specific, allows retrospective AR studies, and offers optimally preserved morphology.


PMID: 8027537

DOI: 10.1177/42.8.8027537

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Schmidt W.E.; Seebeck J.; Hoecker M.; Schwarzhoff R.; Schaefer H.; Fornefeld H.; Morys Wortmann C.; Foelsch U.R.; Creutzfeldt W., 1992: Pacap stimulates enzyme secretion in rat pancreatic acini via interaction with pacap vip receptors and activation of adenylate cyclase differentiation of type 1 pacap and type 2 pacap vip receptors. Gastroenterology 102(4 PART 2): A290

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Arredondo-Peter, R.; Escamilla, E., 1993: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Protein Banding Patterns among Rhizobium leguminosarum biovar phaseoli Strains Isolated from the Mexican Bean Phaseolus coccineus. Several rhizobial strains were isolated from Phaseolus coccineus root nodules and were determined to be Rhizobium leguminosarum biovar phaseoli strains after reinfection of the same host plant. These strains were characterized by cultural procedur...

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Ansell, J.D.; Hodson, B.A.; Woodruff, M.F., 1986: The provenance of cells in sarcomas induced in chimaeric mice. British Journal of Cancer 54(5): 853-855

Narita, A.; Nimura, S.; Ishida, K., 1991: Age-related changes in the activities of cytochrome oxidase and succinate dehydrogenase in mouse oocytes. Oocytes were collected from mice at 30, 60-90 and 100-120 days of age, and their cytochrome oxidase and succinate dehydrogenase activities were determined immediately after collection. All oocytes showed cytochrome oxidase activity; the activity d...

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Woodward, J.A.; Bonett, D.G.; Brecht, M.L., 1985: Estimating the size of a heroin-abusing population using multiple-recapture census. Nida Research Monograph 57: 158-171