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Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pre-treatment


, : Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pre-treatment. Journal of Histochemistry and Cytochemistry 42(8): 1169-1175

We describe the immunohistochemical detection of the human androgen receptor (AR) in routinely processed, paraffin-embedded tissue with the monoclonal antibody (MAb) F39.4. Deparaffinized sections were heated in a microwave oven for antigen retrieval. A panel of human male- and female-derived tissues was investigated. We observed a nuclear staining pattern consistent with previous results on frozen sections. Moreover, we studied the possibility of detecting AR in prolonged formalin-fixed tissue and in paraffin-embedded archival material. After prolonged fixation times or long-term storage of paraffin-embedded tissue, the staining intensity for the AR did not deteriorate. Blocking experiments with the specific synthetic peptides demonstrated the specificity of this technique. We conclude that this method is specific, allows retrospective AR studies, and offers optimally preserved morphology.

US$19.90

PMID: 8027537

DOI: 10.1177/42.8.8027537


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Schmidt W.E.; Seebeck J.; Hoecker M.; Schwarzhoff R.; Schaefer H.; Fornefeld H.; Morys Wortmann C.; Foelsch U.R.; Creutzfeldt W., 1992: Pacap stimulates enzyme secretion in rat pancreatic acini via interaction with pacap vip receptors and activation of adenylate cyclase differentiation of type 1 pacap and type 2 pacap vip receptors. Gastroenterology 102(4 PART 2): A290

Stowe, L.A.; Haverkort, M., 2003: Understanding language. Brain and Language 86(1): 1-8

Arredondo-Peter, R.; Escamilla, E., 1993: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Protein Banding Patterns among Rhizobium leguminosarum biovar phaseoli Strains Isolated from the Mexican Bean Phaseolus coccineus. Several rhizobial strains were isolated from Phaseolus coccineus root nodules and were determined to be Rhizobium leguminosarum biovar phaseoli strains after reinfection of the same host plant. These strains were characterized by cultural procedur...

Ollis D.L.; Brick P.; Hamlin R.; Xuong N.G.; Steitz T.A., 1985: Structure of large fragment of escherichia coli dna polymerase i complexed with deoxy tmp. The 3.3-.ANG. resolution crystal structure of the large proteolytic fragment of E. coli DNA polymerase I complexed with deoxythymidine monophosphate consists of 2 domains, the smaller of which binds Zn-deoxythymidine monophosphate. The most striki...

Offergeld, R., 1982: Cooperation in forest technology in the development policy of the Federal forest administration West Germany.1. Allgemeine Forstzeitschrift, 37(33-34): 991-992

Ansell, J.D.; Hodson, B.A.; Woodruff, M.F., 1986: The provenance of cells in sarcomas induced in chimaeric mice. British Journal of Cancer 54(5): 853-855

Narita, A.; Nimura, S.; Ishida, K., 1991: Age-related changes in the activities of cytochrome oxidase and succinate dehydrogenase in mouse oocytes. Oocytes were collected from mice at 30, 60-90 and 100-120 days of age, and their cytochrome oxidase and succinate dehydrogenase activities were determined immediately after collection. All oocytes showed cytochrome oxidase activity; the activity d...

Gauss J.F.; Taylor G.A., 1969: Environmental factors influencing reproductive differentiation and the subsequent formation of the inflorescence of brassica oleracea var italica d cultivar coastal. Journal of the American Society for Horticultural Science 94(3): 275-280

Gambling, L.; Charania, Z.; Hannah, L.; Antipatis, C.; Lea, R.G.; McArdle, H.J., 2002: Effect of iron deficiency on placental cytokine expression and fetal growth in the pregnant rat. Iron deficiency anaemia is the most common nutritional disorder in the world. Anaemia is especially serious during pregnancy, with deleterious consequences for both the mother and her developing fetus. A model was developed to investigate the mech...

Woodward, J.A.; Bonett, D.G.; Brecht, M.L., 1985: Estimating the size of a heroin-abusing population using multiple-recapture census. Nida Research Monograph 57: 158-171