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Immunohistochemical detection of the type I interferon receptor in human fetal, adult, and neoplastic tissues

, : Immunohistochemical detection of the type I interferon receptor in human fetal, adult, and neoplastic tissues. Modern Pathology 9(2): 150-156

We have used the monoclonal antibody IFNaR3 that recognizes the alpha subunit of the type I interferon (IFN) receptor to study the expression of this receptor in a large series of normal human adult and fetal tissues, as well as in a large number of tumors of diverse origin. Among fetal tissues (8-20 weeks) the type I IFN receptor was expressed in liver, striated muscle, epidermis, renal tubules, choroid plexus of the CNS, and epithelia of different origins (bronchial, gastrointestinal, and pancreatic). Adult tissues showed a similar pattern that includes epithelia from salivary ducts, genital tract, bladder, breast, as well as germinal centers of lymph nodes, tonsils, and spleen. The study of a large series of tumors revealed that the type I IFN receptor is expressed in most, but not all, melanomas, bladder, kidney, small bowel, lung, and breast adenocarcinomas. The majority of lymphomas, sarcomas, and endocrine tumors proved negative. These results support the concept that the type I IFN receptor is rather ubiquitously expressed in normal and malignant epithelial tissues. More interestingly, the expression of the type I IFN receptor was not detected in all tumors, raising the question of whether some cases may fail IFN-alpha therapy due to the lack of receptor expression. This report demonstrates that the IFNaR3 monoclonal antibody can be used for receptor detection in paraffin-embedded sections and it could represent a useful tool in the search for correlations between IFN-alpha response and receptor expression in different diseases.


PMID: 8657722

Other references

Bennell, K.L.; Metcalf, B.; Crossley, K.M.; Buchbinder, R.; Mcconnell, J.; Green, S.; Mccoll, G., 2003: Efficacy of physical therapy for knee joint osteoarthritis A randomised, double-blind, placebo controlled Trial. Medicine & Science in Sports & Exercise 35(5 Supplement): S241, May

Flower., 1865: The note seems to confirm Mr. Flower's more detailed observations P. Z. S. 1860, pp. 333, 334.. The note seems to confirm Mr. Flower's more detailed observations (P. Z. S. 1860, pp. 333, 334).

Chen Y.X.; Welte K.; Gebhard D.H.; Evans R.L., 1984: Induction of t cell aggregation by antibody to a 16 kilodalton human leukocyte surface antigen. A human leukocyte antigen, Leu-13, defined by a mouse monoclonal antibody that reacts with T and B lymphocytes 57 was studied. Optimal staining of T cells requires a much higher concentration of anti-Leu-13 antibody (.alpha.Leu-13) (.gtoreq. 200 ....

Beiseigel, J.M.; Rountree, H.K.; Nickols Richardson, S.M., 2000: Body composition and bone mineral density in moderately active young adult females with normal body mass index. Journal of Bone & Mineral Research 15(Suppl 1): S296, September

Franz, V., 1931: Die Akkommodation des Selachierauges und seine Abblendungsapparate, nebst Befunden an der Retina. Zool. Jena (Allg. Zool.): 323-462

Farrand, W.R., 1990: Quaternary Type Sections. Imagination or Reality? Jim Rose and Christian Schluchter, Eds. Balkema, Brookfield, VT, 1989. viii, 208 pp., illus. $48. From a symposium, Zurich, Switzerland, Oct. 1985. Science 247(4944): 867-867

Suarez J.; Rubin R., 1991: Phenylisopropyl adenosine pia inhibits glycolytic flux g f in isolated rat heart perfused at constant flow. FASEB Journal 5(6): A1572

Frenzel, A.J.; Lui, C.H.; Shin, Y.C.; Kong, J.; Gedik, N., 2014: Semiconducting-to-metallic photoconductivity crossover and temperature-dependent Drude weight in graphene. We investigate the transient photoconductivity of graphene at various gate-tuned carrier densities by optical-pump terahertz-probe spectroscopy. We demonstrate that graphene exhibits semiconducting positive photoconductivity near zero carrier dens...

Gauss, U.; Sander, K., 1966: The stadial dependency of embryonal double formations of Chironomus th. thummi. Die Naturwissenschaften 53(7): 182-183

Hirose, Y., 2005: Discovery of insect parasitism and subsequent development of parasitoid research in Japan. In Japan, early research on insect parasitism, i.e., from 1850 onwards, had a profound effect of sericulture. Insect parasitism in Japan was recognized in 1856, when S. Iimuro first described parasitism of silkworm larvae by the tachinid Blepharip...