+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical diagnosis and significance of forensic neuropathological changes

, : Immunohistochemical diagnosis and significance of forensic neuropathological changes. Journal of Medical Investigation 44(3-4): 109-119

Immunohistochemistry is very useful when investigating the cause of death. Ischemic cell changes in the hippocampal neurons were not obvious in the brains damaged by hypoxic injury. However, it is suggested that even a moderate hypoxia, which may affect the neuronal proteins and metabolism, induced astrocytosis in the CA3 and CA4 regions, and that in patients with a history of hypoxic attacks neuronal damage may be severe even several hours after ischemic injury. Furthermore, hsp70 expression was found in the CA2, CA3 and CA4 regions of long-term survivors after severe hypoxic/ischemic injury. In forensic practice, detailed information about the duration and extent of a hypoxic/ischemic injury is often unavailable, so that immunohistochemical detection of hsp70 and glial cell staining can be of great value in diagnosing not only the hypoxic/ischemic injury during the process of death but also the victim's past history of hypoxic attacks. In diffuse axonal injury, degeneration of axon and myelin, such as swelling and waving, were observed in survivors of more than 8 hours. Retraction balls appeared in survivors of more than 1 days. In longer term survivors, such as 3 or 5 months, breakdown of myelin and fat-granule cells were observed. In addition, retraction balls were also found. Immunohistochemical staining of 200 kD neurofilament was a very useful method to examine axonal changes, because antisera is specific for degenerative neurofilaments. In our study, all cases which had pathological findings of diffuse axonal injury (DAI) were associated with focal head injuries. From the immunohistochemical staining of neurons in the hippocampus, it was suggested that neurons in the hippocampus were injured by diffuse brain damage. Furthermore, repairing and protective mechanisms occurred especially from CA2 to CA4. It was considered that neuronal damage in diffuse brain injury was elucidated not only morphologically but also functionally. Therefore, in cases of suspected diffuse brain damage, it is recommended to examine the neuronal changes in addition to observing the findings of diffuse axonal injury. Immunohistochemical staining of the carotid body is potentially very useful for necropsy diagnosis, since it provides a method to detect evidence of mechanical asphyxia in suspected cases of manual and/or ligature strangulation.


PMID: 9597798

Other references

Arnason, A.; Johannsson, E.; Guomundsson, A.; Dahl, H.A., 1995: Prevention of injuries in Iceland elite soccer players. Medicine & Science in Sports & Exercise 27(5 SUPPL ): S53

Petronievics, B., 1935: Historische Bemerkung iiber die erste Feder der Archaeornithes. The rule of priority makes Archaeopteryx lithographica* the correct appellation for the isolated feather described by von Meyer (1861) from the Jurassic of Bavaria.

Hincks, W.D., 1933: Notes on the Passalidae. EntMag., London: 10-13

Zwicker, H.; Alder, W., 1980: The value of pretherapeutic lymphography in testicular teratoma and carcinoma of the bladder. According to the results of our investigations pretherapeutic lymphography is of high clinical value in cases of testicular teratoma and carcinoma of the bladder. The exstirpation and subsequent histological evaluation of all retroperitoneal lymph...

Stavskaya, S.S.; Krivets, I.A.; Nastoyashchaya, N.I., 1987: Variation in Pseudomonas aeruginosa 1C, an alkyl sulphate degrader. The capacity of P. aeruginosa to destroy alkyl sulphates (surfactants) has sometimes been used to free waste waters of these compounds, but may be affected by the variability of this organism. When P. aeruginosa was incubated in the presence of do...

Wallstabe, L.; Veitt, R.; Körner, T., 2002: Diagnosis of perforated gastric ulcers by ultrasound. Patients with a perforation of the gastrointestinal tract need fast confirmation of diagnosis and early treatment to improve outcome. Plain abdominal x-ray dose not always prove the perforation particularly at early stage. We report about a 62 yea...

Rekila, T.; Harri, M.; Ahola, L., 1997: Validation of the feeding test as an index of fear in farmed blue and silver foxes. The reliability and validity of the eating behaviour in the presence of man (Feeding test) as an index of fear were assessed in fanned blue (Alopex lagopus) and silver foxes (Vulpes vulpes). Repeatability of the Feeding test was good in both speci...

Janda, L.; Durdevic, B.; Dordevic, R., 1968: A contribution to the study of rationalizing plum harvesting. The use of catching frames speeded up plum harvesting 2-3-fold compared with the standard method of shaking the trees and picking up the fruit from the ground.

Anonymous, 1988: Joint meeting of the Swiss Association and the French-Language Society of Electroencephalography and Clinical Neurophysiology. Lausanne (Switzerland), 12-13 June 1987. Abstracts. Neurophysiologie Clinique 18(1): 73-91

Van Den Berg, M.; Ospina, L.; Fajardo, L.F.lipe, 1965: Deficiency of factor XI and coarctation of the aorta in a Colombian patient. The clinical history of a 16 yr, old boy with Factor XI deficiency is presented; the patient also had coarctation of the aorta which was corrected. This is the first description of Hemophilia C (PTA [plasma thromboplastin antecedent] deficiency) i...