geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical differentiation of arteriosclerotic intimal tissue specimen from primary and restenoses


, : Immunohistochemical differentiation of arteriosclerotic intimal tissue specimen from primary and restenoses. Vasa 23(1): 15-22

Smooth muscle cells and macrophages are essential parts of arteriosclerotic lesions. This study should enable the analysis of different functional and morphological features of cells in primary and restenotic biopsies. Therefore detection of specific cell markers in human arteriosclerotic material derived from directional coronary atherectomy (DCA) of primary and restenotic lesions was used. Cryosections (2-4 mu-m) were stained immunohistologically (44 samples from 27 patients, 12 restenoses) with monoclonal antibodies. For further analysis antibodies against 5B5 (Prolylhydroxylase), alpha-actin, desmin and collagen I and IV were used. Endothelial structures (CD 31), T-cells and monocyte derived macrophages (CD 68, KP 1) were detected. In a second set-up alpha-actin and CD 68 were double-labelled. Semi-quantitative analysis in relation to total cell count was performed. The tissue removed consisted quantitatively of 78% intima- and 22% media-compartments and thrombus. 40% of the intimal area was comprised of low cellular compartments and in 57% high cellularity and foam-cells were found. In high cellular compartments 81% of the cells were positive for alpha-actin, only 13% of these cells were also positive for desmin. In intimal compartments in 58% of the cells macrophages were detected by CD-68 marker immunohistologically. 87% of these cells showed the expression of the 5B5 antigen (Prolylhydroxylase), in 90% monocytic antigens were detected. Typical foam cells were not alpha-actin positive, whereas 40% of the CD 68 positive cells were alpha-actin positive. In myofibroblastic cells, positive staining with 5B5, collagen IV and extracellular matrix (collagen I) was present. Staining with antibodies against contractile filaments was diminished compared to the media compartments. Pericellular extensive amounts of extracellular matrix deposits were found there. The results show phenotypic modulations of smooth muscle cells in arteriosclerotic tissue. Basement membranes, extracellular matrix, contractile filaments and 5B5 were distributed differently in media and intima compartments. The antibodies used in the present study enabled the characterization of smooth muscle cells and myofibroblastic cells. The frequently found contractile cells in the arteriosclerotic intima met the criteria of "myofibroblasts". Furthermore, in arteriosclerotic lesions a transformation of cells with macrophage properties from monocytic and myofibroblastic cells seems to be possible.

US$29.90


Other references

Igalffy K., 1980: Swallows exterminated by a pair of little owls athene noctua. Larus (English Translation) 3: 282

Snow, J., 1981: Photographing a heronry. Lincolnshire Trust for Nature Conservation Newsletter, 22 No. 55

Liang, J-Ru.; Li, Z-Ying.; Liu, F-Wu.; Zhou, L-Xiang., 2014: Mineralogical characteristics of biogenic schwertmannite amended with different pretreatment methods and the effects on As(III) absorption. Biogenic schwertmannite has better absorption performance for As(III) than other adsorbents, but there is obvious agglomeration of mineral particles due to the polysaccharides secreted by the bacteria during the synthesis of schwertmannite. The ai...

Ferrer, Julio., 1993: Essai de revision des especes africaines et europeennes appartenant au genre Gonocephalum Solier (Coleoptera, Tenebrionidae). The European and African species of genus Gonocephalun Solier and Opatropis Reitter are reviewed, a new interpretation of genus Gonocephalum is given and Opatropis is degraded to subgenus. A key to 113 hitherto know species and 51 subspecies of sb...

Folco, E.J.; Rocha, V.Z.; López-Ilasaca, M.; Libby, P., 2009: Adiponectin inhibits pro-inflammatory signaling in human macrophages independent of interleukin-10. Macrophages participate pivotally in the pathogenesis of many chronic inflammatory diseases including atherosclerosis. Adiponectin, a vasculoprotective molecule with insulin-sensitizing and anti-atherogenic properties, suppresses pro-inflammatory...

Rosso, P.; Diggs, J.; Winick, M., 1973: Changes in alkaline ribonuclease activity during compensatory renal growth. Unilateral nephrectomy in rats produces an increase in ribonuclease activity in both nuclear and cytoplasmic fractions of the contralateral kidney. The timing of this increase and the changes in the proportion of free activity to protein-bound act...

Abe, A.; Okumura, T., 1972: Influence of aphid infestation on the chemical composition and nutritive value of alfalfa. Samples of leaf, stem and whole plant of lucerne grown in Japan, either infested with aphid or kept aphid-free (control) by use of a pesticide, were analysed. Digestibility of stem cell wall material and whole plant was estimated in vitro with cel...

Guerrero, S.; Alzamora, S.M.; Gerschenson, L.N., 1994: Development of a shelf-stable banana puree by combined factors: microbial stability. A preservation process based on the "hurdle" effect for obtaining a shelf-stable banana puree was developed. The microbial stability of the puree was challenged with inoculation of osmophilic and non-osmophilic yeasts, various molds, Bac...

Dauvillee, D.; Mestre, V.; Colleoni, C.; Slomianny, M.C.ristine; Mouille, G.; Delrue, B.; D'hulst, C.; Bliard, C.; Nuzillard, J.M.rc; Ball, S., 2000: The debranching enzyme complex missing in glycogen accumulating mutants of Chlamydomonas reinhardtii displays an isoamylase-type specificity. To investigate the functions of debranching enzymes in starch biosynthesis, we have partially purified and characterized these activities from wild type and mutant sta7 Chlamydomonas reinhardtii. Mutants of the STA7 locus substitute synthesis of i...

Fredriksson, S.; Banér, J.; Dahl, F.; Chu, A.; Ji, H.; Welch, K.; Davis, R.W., 2007: Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector. Herein we present Gene-Collector, a method for multiplex amplification of nucleic acids. The procedure has been employed to successfully amplify the coding sequence of 10 human cancer genes in one assay with uniform abundance of the final products...