+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical evaluation of dystrophin expression in small round cell tumours of childhood

, : Immunohistochemical evaluation of dystrophin expression in small round cell tumours of childhood. Modern Pathology 6(6): 679-683

Dystrophin, the protein product of the Duchenne muscular dystrophy locus, is a major component of the subsarcolemmal cytoskeleton in skeletal muscle cells and is a relatively muscle specific protein. We evaluated, retrospectively, the expression of dystrophin in 23 small round cell tumors of childhood including: 9 rhabdomyosarcomas, 4 neuroblastomas, 1 ganglioneuroblastoma, 2 small noncleaved cell lymphomas, 1 lymphoblastic lymphoma, 2 medulloblastomas, 2 primitive neuroectodermal tumors, 1 Wilms' tumor, and 1 undifferentiated sarcoma. Frozen sections were stained by the avidin biotin immunoperoxidase technique using a commercially available monoclonal antibody NCL-DYS1 (Novo-castra) that recognizes the mid-rod domain (between amino acids 1181 and 1388) of human dystrophin. Positive controls were represented by frozen sections of normal skeletal muscle. Negative controls consisted of substituting the dystrophin antibody for normal rabbit serum. Eight of nine rhabdomyosarcomas revealed positivity whereas, none of the other tumors stained for dystrophin. This study provides evidence that monoclonal antibodies to dystrophin may be a potential useful addition to the panel of muscle markers used to diagnose small round cell tumors of childhood.


PMID: 7508114

Other references

Bergouignan, M.; Fontan, F.; Trarieux, M.; Julien, J., 1961: Choreiform syndromes in children during cardiosurgical operations under deep hypothermia. Revista de Obstetricia Y Ginecologia de Venezuela 105: 48-60

Verdier, P.C.; Eaton, R.D., 1987: A study of the nutritional status of an Inuit population in the Canadian high arctic. Part 2. Some dietary sources of vitamins A and C. Canadian Journal of Public Health 78(4): 236-239

Webster, P.S.; Oleson, F.B.; Paterson, D.L.; Arkin, C.F.; Mangili, A.; Craven, D.E.; Adcock, D.M.; Lindfield, K.C.; Knapp, A.G.; Martone, W.J., 2008: Interaction of daptomycin with two recombinant thromboplastin reagents leads to falsely prolonged patient prothrombin time/International Normalized Ratio results. A cluster of patients experiencing elevations of International Normalized Ratio without clinical bleeding in temporal association with daptomycin therapy was identified during postmarketing safety surveillance. A common element was the thromboplas...

Schlegel, H.J.; Kreibig, W., 1977: In memory of Wilhelm Kreibig, 10 December 1900-25 June 1976. Klinische Monatsblatter für Augenheilkunde 171(2): 298-300

Makhija, P.; Rameshkumar, K.; Nirmala, V., 2002: Histologic spectrum of struma ovarii. Six cases of struma ovarii, including two with associated carcinoid, with unusual microscopic features are described. The patients' age ranged from 22 to 50 years. The patients were clinically euthyroid. The largest tumour was 10 cms. in grea...

Takamatsu, S.K.; Martens, M.P.; Arterberry, B.J., 2015: Depressive Symptoms and Gambling Behavior: Mediating Role of Coping Motivation and Gambling Refusal Self-Efficacy. Understanding the variables that contribute to the comorbidity of depression and gambling behaviors is important in developing effective intervention strategies for those who experience gambling-related problems. The purpose of this study was to i...

Weisberg, I.; Tran, P.; Christensen, B.; Sibani, S.; Rozen, R., 1998: A second genetic polymorphism in methylenetetrahydrofolate reductase (MTHFR) associated with decreased enzyme activity. A common mutation in methylenetetrahydrofolate reductase (MTHFR), C677T, results in a thermolabile variant with reduced activity. Homozygous mutant individuals (approximately 10% of North Americans) are predisposed to mild hyperhomocysteinemia, wh...

Colten, S.I., 1978: Community residential treatment strategies. Community Mental Health Review 3(5-6): 1, 16-1, 21

Lapirov Skoblo, S.Ja., 1968: Forest products. An official Soviet textbook in two parts, one describing the classification and standardization of forest products, and the other describing the products themselves and their specifications, types, quality, etc. The range of products covers roundw...

Dyson, J.; Cowdell, F., 2015: Development and psychometric testing of the 'Motivation and Self-Efficacy in Early Detection of Skin Lesions' index. To develop and psychometrically test the Motivation and Self-Efficacy in Early Detection of Skin Lesions Index. Skin cancer is the most frequently diagnosed cancer worldwide. The primary strategy used to prevent skin cancer is promotion of sun avo...