geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical examination of the expression of O-6-methylguanine-DNA methyltransferase in human melanoma metastases


, : Immunohistochemical examination of the expression of O-6-methylguanine-DNA methyltransferase in human melanoma metastases. European Journal Of Cancer. 33(1): 129-134

In tumour cell lines, an inverse relationship has been shown between susceptibility to the cytotoxic effects of the O-6-alkylating agents and the expression of the DNA repair protein O-6-methylguanine-DNA methyltransferase (MGMT). One of the most effective single agents in chemotherapy of metastatic melanoma is the O-6-alkylating drug, dacarbazine. We therefore examined the distribution of MGMT in 37 skin and lymph node melanoma metastases using rabbit antihuman MGMT antiserum. MGMT expression was undetectable in tumours from 2 out of 34 patients and low in 4 further patients. When present, staining was mainly nuclear and showed a marked variation both among tumour cells within the same metastases, between separate metastases in the same patient and between tumours in different patients. MGMT expression determined by immunohistochemistry showed a relation to MGMT activity measurements, but was not related to the number of proliferating cells, as identified by staining with MIB-1 antibody. Tumour cells with moderate to strong immunostaining with MGMT antiserum were significantly more abundant in metastases excised after dacarbazine-based chemotherapy (n = 8) than in those excised before treatment (n = 29).

US$19.90

PMID: 9071912

DOI: 10.1016/s0959-8049(96)00342-5


Other references

Teramoto, M.; Nakamasu, K.; Noshiro, M.; Matsuda, Y.; Gotoh, O.; Shen, M.; Tsutsumi, S.; Kawamoto, T.; Iwamoto, Y.; Kato, Y., 2001: Gene structure and chromosomal location of a human bHLH transcriptional factor DEC1 x Stra13 x SHARP-2/BHLHB2. DEC1/BHLHB2 is a novel cAMP-inducible basic helix-loop-helix (bHLH) transcriptional factor isolated from human chondrocyte cultures by the subtraction method [Shen et al. (1997) Biochem. Biophys. Res. Commun. 236, 294--298]. DEC1 seems to be invol...

Eskinja, M.; Zoellner, P.; Linnemayr, K.; Mak, M.; Schmid, E.R., 1997: Characterization of mercapturic acids by three different mass spectrometric methods. The determination of mercapturic acids, as metabolic end-products of an important pathway in the biotransformation of xenobiotics, in human urine is becoming a standard tool for the biomonitoring of populations exposed to potential mutagens and ca...

Cobb M.; Burnet B.; Connolly K., 1986: The structure of courtship in the drosophila melanogaster species sub group. A quantitative comparison and sequence analysis of courtship behaviour of four-day old virgin flies from seven species of the Drosophila melanogaster species subgroup is described. In three species (D. melanogaster, D. simulans and D. mauritiana)...

Ricker, M.; Daly, D.C.; Veen, G.; Robbins, E.F.; Sinta V.M.; Chota I.J.; Czygan, F.C.; Kinghorn, A.D., 1999: Distribution of quinolizidine alkaloid types in nine Ormosia species (Leguminosae-Papilionoideae). Quinolizidine alkaloids were surveyed in 22 plant samples, representing nine Ormosia species from Mexico and Peru, and up to five different plant parts (seeds, fruits, bark, roots, leaves) per species, using combined gas chromatography and mass sp...

Chidambaram, J.D.; Melese, M.; Alemayehu, W.; Yi, E.; Prabriputaloong, T.; Lee, D.C.; Cevallos, V.; Zhou, Z.; Whitcher, J.P.; Gaynor, B.D.; Lietman, T.M., 2004: Mass antibiotic treatment and community protection in trachoma control programs. Azithromycin is highly effective against trachoma, but the practical difficulties of community-wide distribution often leave many individuals untreated. We demonstrate, after mass azithromycin treatment of a population in Ethiopia, an indirect pro...

Simon, B.I.; Chiang, T.Fai.; Drew, H.J., 2010: Alternative to the gold standard for alveolar ridge augmentation: tenting screw technology. Studies have discussed the potential issues associated with the placement of implants in unfavorable locations. Today, it is possible to optimize implant placement despite the significant ridge resorption that often occurs after tooth extraction....

Levine Richard M.; Bond Andrew R., 1994: Tin reserves and production in the Russian Federation. International Geology Review 36(3): 301-310

Singh L.A.K., 1983: Observations on food requirement and food conversion in the mugger crocodylus palustris reared in captivity. Journal Of The Bombay Natural History Society: 418-423

Todd, C.S.; Nasir, A.; Stanekzai, M.R.; Scott, P.T.; Close, N.C.; Botros, B.A.; Strathdee, S.A.; Tjaden, J., 2011: HIV awareness and condom use among female sex workers in Afghanistan: implications for intervention. There is little information about HIV awareness or condom use among female sex workers (FSWs) in Afghanistan. The purpose of this cross-sectional study was to assess HIV awareness, knowledge, and condom use among FSWs in three Afghan cities. FSWs...

Winokur, P.L.; McLinden, J.H.; Stapleton, J.T., 1991: The hepatitis A virus polyprotein expressed by a recombinant vaccinia virus undergoes proteolytic processing and assembly into viruslike particles. Hepatitis A virus (HAV) contains a single-stranded, plus-sense RNA genome with a single long open reading frame encoding a polyprotein of approximately 250 kDa. Viral structural proteins are generated by posttranslational proteolytic processing of...