+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical expression of P53 tumor suppressor gene protein in adult germ cell testis tumors: clinical correlation in stage I disease

, : Immunohistochemical expression of P53 tumor suppressor gene protein in adult germ cell testis tumors: clinical correlation in stage I disease. Journal of Urology 152(2 Pt 1): 418-423

P53 tumor suppressor gene protein immunostaining was evaluated in the primary tumor of adult testicular germ cell cancer to assess if P53 expression would serve as a clinically useful tumor marker. Representative archival tissues from 152 orchiectomy specimens were studied for P53 immunohistochemistry. Seminoma and nonseminomatous germ cell tumor constituents revealed P53 expression via immunohistochemistry in 90% and 94% of the cases, respectively. For seminoma, there was a trend toward decreased P53 expression with advancing stage. For nonseminomatous germ cell tumor, although all cellular components showed variable P53 expression, P53 expression in embryonal carcinoma constituents increased among stages of disease. A third of pathological stage I cancer patients exhibited 2+ or greater P53-embryonal staining compared with 61% with stage II (p = 0.0670) and 67% with stage III (p = 0.0815) disease, respectively (Kruskal-Wallis, 2-sided test). As a secondary objective, we wanted to determine if P53 immunohistochemistry would be useful to predict occult disease in clinical stage I nonseminomatous germ cell tumor. This group was studied for P53-embryonal immunohistochemistry, the presence of vascular invasion and the quantitative determination of percentage of embryonal carcinoma in the primary tumor in a multivariate fashion to assess if these tests could be clinically useful to predict occult disease. Degree of P53 immunostaining of the embryonal component in the primary tumor was statistically greater for stage II by univariate logistic regression analysis (p = 0.0362). Similarly, the per cent embryonal cancer (p = 0.0002) and vascular invasion (p = 0.0005) were highly significant as predictors of occult stage II disease via the univariate testing. By multivariate logistic regression analysis, the model consisting of per cent embryonal cancer and vascular invasion provided the best prediction of occult disease in the clinical stage I cohort. In addition, this model had the highest sensitivity and specificity of all multivariate models considered. The addition of P53-embryonal staining did not improve predictability nor sensitivity/specificity. The P53 tumor suppressor gene protein is expressed to some degree in most testicular germ cell tumors and degree of staining/expression varies according to stage of disease. From the standpoint of a clinically useful primary tumor risk factor for predicting occult disease, vascular invasion by the tumor and percentage of embryonal carcinoma component in the tumor are more useful than P53 immunohistochemistry.


PMID: 8015085

Other references

Helm, Jl, 1992: 1992 North Dakota Wheat variety performance descriptions. NDSU Extension Service ication North Dakota State University: 92 (A-574)

Krawczuk-Robak, A., 1989: Topography and cytoarchitectonics of the nuclei of the posterior part of hypothalamus in insectivora. Part II. Regio premamillaris. Cross-section paraffin scraps were stained with the methods of Nissl and Klüver-Barrera. A description was given of the topography, shape and cell structure of the nuclei in the regio premamillaris in 3 insectivorous mammals: hedgehog, mole and c...

Munir, K.M.; Lamos, E.M., 2017: Diabetes type 2 management: what are the differences between DPP-4 inhibitors and how do you choose?. Expert Opinion on PharmacoTherapy 18(9): 839-841

Opini, O.N. 1016 ., 1974: Crlbrilina punctata (Hassan, 1841): designation of a neotype under the Plenary Powers. Bulletin of Zoological Nomenclature, 311: 19-21

Berry Edward Wilber, 1912: The age of the plant-bearing shales of the Richmond coal field. American Journal of Science: ages 224-225. 1912.

Newton, E.T., 1888: Notes on Pterodactyls. A review of our knowledge of this order.

Ma, Z-Ying.; Qiao, X.; Xie, C-Zhi.; Shao, J.; Xu, J-Yuan.; Qiang, Z-Yan.; Lou, J-Shi., 2013: Activities of a novel Schiff base copper(II) complex on growth inhibition and apoptosis induction toward MCF-7 human breast cancer cells via mitochondrial pathway. In this study, we investigated the newly synthesized Schiff base copper(II) complex, [Cu(II)(5-Cl-pap)(OAc)(H(2)O)]·2H(2)O (1) (5-Cl-pap=N-2-pyridiylmethylidene-2-hydroxy-5-chloro-phenylamine), inducing growth inhibition and apoptosis in human br...

Tseng,; Wang, X., 2011: Do convergent ecomorphs evolve through convergent morphological pathways? Cranial shape evolution in fossil hyaenids and borophagine canids Carnivora, Mammalia. Cases of convergent evolution, particularly within ecomorphological contexts, are instructive in identifying universally adaptive morphological features across clades. Tracing of evolutionary pathways by which ecomorphological convergence takes pl...

Simon F.; Sutherland E., 1980: Composition of liver surface membrane fractions and the biosynthesis of sodium potassium atpase following pheno barbital administration. Clinical Research 28(2): 485A

Ruiz Carranza, P.M.; Lynch, J.D., 1996: Ranas Centrolenidae de Colombia 9. Dos nuevas especies del suroeste de Colombia. Lozania o 30; 68: 1-11