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Immunohistochemical identification of neurons in ganglia of the guinea pig sphincter of Oddi


, : Immunohistochemical identification of neurons in ganglia of the guinea pig sphincter of Oddi. Journal of Comparative Neurology 352(1): 106-116

The sphincter of Oddi is a smooth muscle sphincter that regulates the flow of bile into the duodenum. To identify potential chemical coding in sphincter of Oddi neurons, immunohistochemistry and histochemistry were employed to assay for putative neurotransmitters and related synthetic enzymes in wholemount preparations, with and without colchicine treatment. Immunoreactivities for enkephalin-endorphin (ENK-END), substance P (SP), nitric oxide synthase, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) were demonstrated within the ganglionated plexus. Roughly half of the neurons in the sphincter of Oddi expressed immunoreactivity for both SP and ENK-END, but not for nitric oxide synthase. About 25% of the neurons expressed nitric oxide synthase immunoreactivity as well as NADPH-diaphorase activity. This contingent of neurons was made up of two subgroups: one that expressed immunoreactivity for VIP, the other for NPY. Neurons that expressed CGRP immunoreactivity were sparse in sphincter of Oddi ganglia; however, many axons immunoreactive for both CGRP and SP were present in the ganglionated plexus. The CGRP/SP fibers are probably visceral afferents that may influence ganglionic output through axon reflex circuits. These results, along with studies of the actions of these neuroactive compounds on sphincter tone, support the view that ganglia of the sphincter of Oddi are largely comprised of excitatory (SP/ENK-END-immunoreactive) and inhibitory (nitric oxide synthase/VIP- or NPY-immunoreactive) neurons, and that sphincter of Oddi tone is controlled by the regulation of the outputs of these two groups of cells.

US$19.90

PMID: 7536219

DOI: 10.1002/cne.903520108


Other references

Andersson, H., 1976: Effects of a fat-reduced diet on the faecal excretion of radioactivity following administration of 14C-cholic acid and on the duodenal concentration of bile salts in patients with ileal disease. The fraction of faecal radioactivity excreted after intravenous administration of 14C-labelled cholic acid decreased in most patients with ileopathy and bile salt malabsorption when the dietary daily fat content was reduced from 100 to 40 g. The d...

Sandermann, H., J.; Addona, G.H.; Miller, K.W., 1997: A thermodynamic analysis of the partitioning of cholesterol and related compounds between trioleoylglycerol and egg phosphatidylcholine bilayers. The free energy of transfer of a number of alcohols, including cholesterol, from a bulk isotropic lipid phase, trioleoylglycerol (TG), to an anisotropic lipid phase, egg phosphatidylcholine (PC), was determined. n-Alkane-1-ols partitioned preferen...

Amin, O.M.; Heckmann, R.A.; Inchausty, V.; Vasquez, R., 1996: Immature Polyacanthorhynchus rhopalorhynchus in Venton, Hoplias malabaricus from Moca Vie River, Bolivia, with notes on its apical organ and histopathology. Cystacanths of Polyacanthorhynchus rhopalorhynchus (Diesing, 1851) Travassos, 1920 were discovered in the viscera of venton, Hoplias malabaricus (Bloch, 1794) collected from the Moca Vie River at Las Palquitas, Bolivia. Apical proboscis organ and...

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QiuHongYan; HouLiYan; ZhaoYongXian(etal), 2010: Analysis on health seeking behaviors of young women in floating population. Objective: To investigate the self-report status, health seeking behaviors and effect factors of gynecologic symptoms among young women in floating population. Methods: 3 521 female floating staff aged 18-29 years old from 10 factories in 6 distri...

Parajuli, D.Ram.; Park, E-Jeon.; Che, X-Hua.; Jiang, W-Yi.; Kim, Y-Chul.; Sohn, D.Hwan.; Lee, S.Hee., 2013: PF2401-SF, standardized fraction of Salvia miltiorrhiza, induces apoptosis of activated hepatic stellate cells in vitro and in vivo. During the course of our attempts to develop a potential herbal medicine, we had previously prepared PF2401-SF, a standardized fraction of S. miltiorrhiza, and reported its hepatoprotective activity in vitro as well as in vivo. Since apoptosis of...

Londono de Hoyos, Concha., 1996: Systematics of benthic foraminifera of Gomez coastal area, Gorgona island Sistematica de foraminiferos bentonicos de playa Gomez isla Gorgona. Acta Biologica Colombiana, 31: 65-78

Dunn, P.M.; Hine, L.C.; Macgregor, M.E., 1964: Search By Clinical Methods For Persistent Urinary Infections In Children. British Medical Journal 1(5390): 1081-1084

Schmutzenhofer, H., 1979: The dark-brown pine leaf beetle (Luperus pinicola) as a pest of Norway spruce. An outbreak of L. pinicola occurred in hills near Pottenstein-Berndorf, S. of Vienna, Austria, from 1965 to 1969. The needles on young shoots of Norway spruce were severely damaged, from Mid-May to the end of June, in pole stands and a 3-yr-old pl...

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