+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical identification of type B intercalated cells in the rat kidney by a monoclonal antibody

, : Immunohistochemical identification of type B intercalated cells in the rat kidney by a monoclonal antibody. Archives of Histology and Cytology 61(2): 151-161

We recently produced monoclonal antibodies using macrophagic cells derived from cultured rat glomeruli as the antigen. One of the antibodies, named OS-3, was found to detect a cell population scattered in collecting ducts of the rat kidney as well as macrophages in various tissues. The present study deals with the cellular and subcellular localization of immunoreactivities with OS-3 in the kidney and other organs of rats. Double immunostaining using OS-3 and an anti-serum against either calbindin or epidermal cytokeratin showed that OS-3-immunoreactive cells exist exclusively in both the connecting segment and cortical collecting duct, and differ from calbindin- or cytokeratin-positive epithelial cells. Ultrastructurally, OS-3-immunoreactive cells appeared spherical in shape with few cytoplasmic microprojections on the narrow apical surface. Their relatively dark cytoplasm contained numerous mitochondria and a developed tubulo-vesicular system. The intense immunoreactivity was selectively localized in the basolateral membrane exhibiting shallow but complicated infoldings. Distribution and ultrastructural properties of the OS-3-immunoreactive cells showed that they were type B intercalated cells, which are engaged in the regulation of the acid-base balance mainly by secreting HCO3-. Another positive staining with OS-3 was found in the macula densa and some epithelial cells of Bowman's capsule, the former monitoring Cl- concentrations in the urine. Immunoblotting of extracts from the rat kidney demonstrated a protein band immunoreactive to OS-3 at a molecular weight of 43 kDa. Aside from the kidney, a specific and intense immunoreactivity with OS-3 was also found in the epithelial cells of the pancreatic excretory duct and in the secretory cells of the salivary, pyloric and duodenal glands, all of which are HCO3- -secreting cells. These immunohistochemical findings imply that OS-3 is useful for the detection of type B intercalated cells and recognizes a functional molecule involved in the production/secretion of HCO3- or transport of Cl-.


PMID: 9650889

DOI: 10.1679/aohc.61.151

Other references

Gava, A., 1999: Lung cancer radiation treatment in the elderly. Critical Reviews in Oncology/Hematology 32(1): 45-48

Mizugami, T.; Mikata, A.; Hajikano, H.; Asanuma, K., 1988: Childhood lymphoma. A clinicopathological and immunohistological study of 58 cases. Fifty-eight cases diagnosed as malignant lymphoma in patients younger than 15 years between 1976 and 1986 in the Kanto area were reviewed and reclassified as follow: 48 non-Hodgkin's lymphomas, 9 Hodgkin's disease and one malignant histi...

Thomas, R.H., 1992: The imaging of impotence. Western Journal of Medicine 156(3): 299-300

Parke, W.W., 1956: Bronchial diverticula in Blarina brevicauda. Journal of Mammalogy, 37: 236-245

Tenesa, A.; Knott, S.A.; Ward, D.; Smith, D.; Williams, J.L.; Visscher, P.M., 2003: Estimation of linkage disequilibrium in a sample of the United Kingdom dairy cattle population using unphased genotypes. The association between genetic marker alleles was estimated for two regions of the bovine genome from a random sample of 50 young dairy bulls born in the United Kingdom between 1988 and 1995. Microsatellite marker genotypes were obtained for six...

Rao, S.R.; Seshadri, C., 1969: Meiotic chromosomes in the male slender loris, Loris tardigradus lydekkarianus (Cabrera). Experientia 25(4): 422-423

Vichyanond, P.; Thakerngpol, K.; Susiva, C.; Chavalittamrong, B., 1993: Ultrastructural defects of the ciliary epithelium in a child with Kartagener's syndrome. Kartagener's syndrome is a well known classical triad of presentations consisting of bronchlectasis, sinusitis and situs inversus. It is now recognized that the syndrome is an extreme presentation of primary ciliary dyskinesia, a large group...

Fafarman, K.R.; Baker, B.W., 1979: Hunger and activity levels of cottontail rabbits. Activity level was taken as the number of rabbits seen in a headlight beam on a 25.4-km journey started at 1900 h or 2100 h during November and December 1976 in Texas. Hunger (stomach fullness) was estimated as the volume of food in the stomach di...

Ismail, F.M.; Dascombe, M.J.; Carr, P.; North, S.E., 1996: An exploration of the structure-activity relationships of 4-aminoquinolines: novel antimalarials with activity in-vivo. The structure-activity relationships of bisquinolines, a potentially important group of novel antimalarial drugs, were studied. The high-temperature (180-250 degree C) synthesis of 4-aminoquinolines, including bisquinolines, by nucleophilic displa...

Shingu, Y.; Kondo, S.; Hata, H., 2010: Differences in grazing behavior of horses and cattle at the feeding station scale on woodland pasture. In this study, grazing behavior of mares and cows was compared on woodland pastures, in the context of foraging hierarchy. A horse-group (12 native Hokkaido horses) and a cattle-group (5 Hereford cows) were grazed on the woodland pastures of 13.3...