+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunohistochemical investigation on intraneural localization of herpes simplex virus type 1 in experimental retinitis. 1. Observation of the retina and optic nerve on the injected side

, : Immunohistochemical investigation on intraneural localization of herpes simplex virus type 1 in experimental retinitis. 1. Observation of the retina and optic nerve on the injected side. Nippon Ganka Gakkai Zasshi 97(4): 474-482

In order to examine transfer of herpes simplex virus type 1 (HSV-1) in the retina and optic nerve in rats with experimental retinitis, an HSV-1 suspension (Miyama strain, 1 x 10(7) plaque-forming units per ml, 10 microliters) was inoculated into the vitreous body. One to seven days after HSV-1 inoculation, the animals were perfused through the heart with Zamboni's fixative. Fifty-microns-thick cryostat-cut sections through the retina and optic nerve were immunostained for HSV-1 antigen with rabbit anti-HSV-1 antiserum and avidin-biotin-peroxidase complex. Some sections were counterstained with hematoxylin. At an early stage of the postinoculation period, HSV-1 immunoreactivity was first seen in the cytoplasm of Müller cells and then in various cells of the inner nuclear layer. HSV-1 immunoreactivity was extremely dense in the nuclei of these cells, suggesting that HSV-1 replicates in the nuclei and propagates through the cytoplasm to the membrane of cell processes. At a later stage of the postinoculation period, HSV-1 immunoreactivity was seen in some retinal ganglion cells and optic nerve fibers. The labeling of the optic nerve fibers was found even in the optic tract and often accompanied by labeled glial cells adjacent to the nerve fibers.


PMID: 8391206

Other references

Wiercińska-Drapało, A.; Tarasów, E.; Jaroszewicz, J., 2003: The central nervous system and HIV infection. Human immunodeficiency virus (HIV) may be the cause of both primary and secondary brain diseases. In this review general features of HIV-associated neuropathology are discussed. Up to 90% of patients with AIDS have a variety of HIV-related brain d...

McLean, D.L.; Kinsey, M.G., 1968: Probing behavior of the pea aphid, Acyrtho siphon pisum. 3. Effect of temperature differences on certain probing activities. Annals of the Entomological Society of America, 61: 927-933

Lee, D.B., 1964: Wastes-Wastes-Wastes. Journal of the Florida Medical Association. Florida Medical Association 51: 297-298

Knipple, D.C.; Seifert, E.; Rosenberg, U.B.; Preiss, A.; Jäckle, H., 1985: Spatial and temporal patterns of Krüppel gene expression in early Drosophila embryos. The Krüppel (Kr) locus is a member of the 'gap' class of segmentation genes of Drosophila melanogaster. Mutations at the Kr locus cause the deletion of contiguous segments from the embryonic body pattern. We have elucidated the spatial...

Kittler, P.M.; Phan, H.T.T.; Gardner, J.M.; Miroshnichenko, I.; Gordon, A.; Karmel, B.Z., 2010: Auditory brainstem evoked responses in newborns with Down syndrome. Auditory brainstem evoked responses (ABRs) were compared in 15 newborns with Down syndrome and 15 sex-, age-, and weight-matched control newborns. Participants had normal ABRs based upon values specific to 32- to 42-weeks postconceptional age. Alt...

MacEwan, D.J., 2015: TWEAKing for a fight with GVHD. In this issue of Blood, Chopra et al provide convincing evidence that tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) ligand acting through its receptor, fibroblast growth factor-inducible 14 (Fn14), is crucial to the intestinal...

Chuvashov B.I., 1996: Devonian-Carboniferous-Permian organic buildups as dynamic indicators of tectonics and sedimentary development in the Urals. Abstracts - Geological Society of Australia 41: 89

Mishra, A.; Deen, V.; Slaughter, R.; Walters, D.L., 2011: Pulmonary vein stenosis: a complication of atrial fibrillation ablation treated with stenting. Heart, Lung & Circulation 19(2): 97-99

Rawlinson, W.D.; Basten, A.; Hargrave, J.C., 1987: Clinical significance of changes in serum proteins, immunoglobulins, and autoantibodies in leprosy. Changes in the level of acute phase reactants such as C-reactive protein (CRP), serum globulins, and autoantibodies have been reported previously in patients with leprosy, particularly at the lepromatous end of the spectrum. The clinical significa...

Helwig, C.C.; Jasiobedzka, U., 2001: The relation between law and morality: children's reasoning about socially beneficial and unjust laws. This study investigated children's reasoning about laws and legal compliance. A total of 72 children, 24 each at 6, 8, and 10 years of age, made judgments of law evaluation ("Is it a good or bad law?"), legitimacy of legal regulatio...