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Immunohistochemical localisation of six glutathione S-transferases within the nasal cavity of the rat

, : Immunohistochemical localisation of six glutathione S-transferases within the nasal cavity of the rat. Archives Of Toxicology. 69(2): 91-98

Many xenobiotics induce lesions within the nasal cavity of experimental animals which are site specific. This site selectivity may be due to regional deposition within the nasal cavity and/or the localization of biotransformation enzymes. We have developed methodology which allows immunohistochemical localization of xenobiotic biotransformation enzymes in transverse sections of the rat nasal cavity identical to those normally taken for pathological examination. We report the application of this methodology to six isoenzymes of the glutathione S-transferases (GSTs). All six isoenzymes were predominantly located within olfactory epithelium covering the ethmoturbinates (levels III and IV) and extending forwards into the dorsal meatus (level II). Squamous and transitional epithelia showed little or no staining while respiratory epithelium was weakly stained. Within the respiratory epithelium only the ciliated columnar cells and, to a lesser extent, some of the seromucous glands contained GSTs. Within olfactory epithelium the sustentacular cells, basal cells and subepithelial glands all stained positive for GSTs. The different cell types of olfactory epithelium preferentially express different GST isoenzymes: 1-1 and 2-2 were predominantly located in the subepithelial glands; 3-3, 4-4 and 8-8 in sustentacular and basal cells; 7-7 in basal cells.


PMID: 7717867

DOI: 10.1007/s002040050143

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Low, C.M.; Zheng, F.; Lyuboslavsky, P.; Traynelis, S.F., 2000: The apparent high-affinity zinc inhibition of NR1/NR2A receptor is due to enhancement of proton inhibition. Recent studies have shown that NMDA receptors comprised of NR1/NR2A are highly sensitive to extracellular zinc, with an apparent IC50 in the nanomolar range. However, the mechanism by which zinc inhibits NR1/NR2A receptors is not well delineated....

Fisher, J.D.; Kim, S.G.; Ferrick, K.J.; Roth, J.A., 1994: Programmed ventricular stimulation using tandem versus simple sequential protocols. The objective was to determine whether two commonly used ventricular stimulation protocols, one more complex than the other, produced concordant results. If such were the case, the simpler protocol would streamline activities in clinical electroph...

James K.Russell, 1978: Effects of interspecific dominance among egrets commensally following roseate spoonbills. The Auk 95(3): 608-610

Ba-Thein, W.; Inui, S.; Shimizu, T.; Swe, T.; Banu, S.; Ohtani, K.; Oe, M.; Sakurai, N.; Nakamura, S.; Hayashi, H., 1997: Genomic diversity in the pfoA region of the theta-toxin-deficient strains of Clostridium perfringens. The genomic structure of the pfoA-colA region in six theta-toxin-deficient strains of Clostridium perfringens was examined by Southern hybridization using the pfoR, pfoA, pbg, arcABDC and colA genes, encoding regulator for pfoA, theta-toxin, beta-...

Ruijs, S.H., 1979: A simple procedure for patient preparation in abdominal CT. Ajr. American Journal of Roentgenology 133(3): 551-552

Graoev, N.K., 1963: Digestive ability, nitrogen metabolism and carcass composition in pigs differing in time of maturity.. A series of experiments was carried out with 12 Murom early-maturing piglings and 12 Murom latematuring piglings. To 6 mths. of age the digestive ability of the former was better than that of the latter; after 6 mths., digestive ability was not ap...

Ewen, L.M., 1973: Synthesis of Cibachron blue F3GA-amylose with increased sensitivity for determination of amylase activity. Clinica Chimica Acta; International Journal of Clinical Chemistry 47(2): 233-248

Namihisa, T.; Kuroda, H.; Imanari, H., 1983: Detection of mitochondria in bile canaliculi in early stage primary biliary cirrhosis cases. Biopsy specimens of liver obtained from patients with early stages of primary biliary cirrhosis were examined by electron microscopy with special attention being paid to changes in bile canaliculi, as well as bile ducts and hepatocytes. Ultrastruc...

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Swift, D.G.; Guillard, R.R.L., 1977: Diatoms as tools for assay of total b 12 activity and cyano cobalamin activity in sea water. The total cobamide concentration (vitamin B12 plus B12 analogs active for some organisms) can be determined in sea water by bioassay utilizing growth of the centric diatom Bellerochea polymorpha clone 675-d. Vitamin B12 activity can be determined...