+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol

, : Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol. Journal Of Histochemistry & Cytochemistry. 44(4): 389-392

Recently, a peroxidase-mediated amplification system has been described for immunofluorescence and fluorescence in situ hybridization studies. It is based on the deposition of hapten- or fluorochrome-labeled tyramide molecules. Although providing a significantly increased detection sensitivity compared to conventional procedures, its localization properties are inferior because of free diffusion of intermediate reaction products before they are immobilized. In enzyme cytochemistry, it is well established that improved localization of enzyme activity can be achieved through the addition of viscosity-increasing polymers to the incubation media. In this study we analyzed the effect of different polymers on the localization sharpness and sensitivity of the tyramide-peroxidase reaction in FISH applications. Significantly improved localization of the fluorescent endproduct was observed using dextran sulfate or polyvinylalcohol (PVA) with, respectively, no or little loss of sensitivity.


PMID: 8601698

DOI: 10.1177/44.4.8601698

Other references

Rosoff, A.J., 1985: Is health care still a basic right in the U.S.?. Group Practice Journal 34(3): 31-33

Zoeller M., 1991: Tnp responsiveness of anti tnp sp6 transgenic mice. Immunobiology 183(3-4): 345

Eibel, P., 1961: Trigger Wrist with Intermittent Carpal Tunnel Syndrome: A Hitherto Undescribed Entity with Report of a Case. Canadian Medical Association Journal 84(11): 602-605

Thompson, P.B., 1992: Designing animals: ethical issues for genetic engineers. Two general philosophical approaches to ethical issues in property rights are described. Instrumental approaches take property rights to be means for achieving goals such as social efficiency or economic growth. Labor approaches take property righ...

Schrum L.W.; Hassan H.M., 1990: Role of dna topology in the expression of the sod a gene in escherichia coli using operon fusion. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 166

Su, H.; Wu, P.; Wang, H.; Chen, Y.; Bao, X., 2014: Design on tester of pull-out force for orthodontic micro implant. A special device for measuring the pull-out force of orthodontic micro implant was designed, which has the characteristics of simple construction and easy operation, and can be used to detect the pull-out-force of orthodontic micro implant. The te...

Damberg, B.; Are, R.; Kliavinia, S., 1976: Preservation of L lysine under different temperatures of drying. Mikrobiologicheskie preparaty: 118-123

Gleghorn, T., 1998: Porcelain laminate veneers to restore worn lower teeth. Dentistry Today 16(9): 46, 48, 50-1

Kim, N.; Yoo, W.; Lee, J.; Kim, H.; Lee, H.; Kim, Y-S.; Kim, D-U.; Oh, J., 2009: Formation of vitamin A lipid droplets in pancreatic stellate cells requires albumin. Quiescent pancreatic stellate cells (PSCs) store vitamin A as cytoplasmic lipid droplets, and, when activated by profibrogenic stimuli, they transform into myofibroblast-like cells characterised by the loss of vitamin A droplets. Activation of ste...

Headstrom, B.R., 1928: An Ancient Walrus Skull. Monophenol oxidase (or tyrosinase-A(1)) activity appears to be absent from extracts from pupae of homozygous lozenge-glossy (lz(g)/lz(g)) females and (lz(g)/male symbol) males of Drosophila melanogaster. Diphenol oxidase (tyrosinase-A(2)) activity...