+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibition of human neutrophil functions by sulfated and nonsulfated cholecystokinin octapeptides

, : Inhibition of human neutrophil functions by sulfated and nonsulfated cholecystokinin octapeptides. Peptides (tarrytown). 18(3): 415-422

The effects of CCK-8s and desulfated CCK8 at concentrations ranging from 10- fwdarw -14 to 10-6 M were studied in vitro on several functions of human peripheral neutrophils: adherence to substrate, mobility (spontaneous and directed by a chemical gradient or chemotaxis), ingestion of inert particles (latex beads) or cells (Candida albicans), and production of superoxide anion measured by the nitroblue tetrazolium reduction test. The effect of CCK-8s on intracellular levels of cAMP was investigated as well as the implication of calcium in the action of CCK-8s on phagocytic function using stimulants and inhibitors of both intracellular and extracellular calcium channels. The two peptides, at concentrations from 10-12 to 10-8 M, inhibited significantly both mobility and ingestion capacities and increased adherence to substrate. A dose-response relationship was observed with a maximum inhibition of neutrophil functions at 10-10 M. CCK-8s and desulfated CCK-8 induced in these cells a significant, but transient, increase of cAMP levels at 60 s. Moreover, CCK-8s was found to inhibit completely the stimulation of latex bead phagocytosis in neutrophils produced by the calcium ionophore A23187. These results suggest that CCK-8 is a negative modulator of several neutrophil functions and that the inhibition of these activities could be carried out through an increase of the intracellular cAMP levels and a decrease of the extracellular calcium input.


PMID: 9145430

DOI: 10.1016/s0196-9781(96)00338-5

Other references

Diel, I.J., 1991: Current possibilities in the therapy and prevention of bone metastases in breast carcinoma?. Zentralblatt für Gynakologie 113(19): 1023-1024

Duronslet M.J.; Revera D.B.; Franklin L.E., 1981: Degenerative changes in the testes of the golden hamster. Journal of Andrology 2(1): 34

Pimentel, G.; Poore, E.R.; Nathanielsz, P.W., 1989: The effect of continuous or pulsatile administration of oxytocin to ewes at 126 to 136 days' gestation on myometrial activity and fetal oxygenation. Oxytocin was infused either continuously or in pulses to pregnant ewes to compare the responses of myometrial activity (contractures) and changes in fetal arterial PO2. Group 1 (n = 5) was infused with saline solution throughout the experiment. In...

Sadeghi, S.E.; Ahmadi, S.M.; Shayesteh, N.; Alizadeh, M.H.; Pourmirza, A., 2004: Study on biology of alder brown leaf beetle, Galerucella lineola (Col.: Chrysomellidae) in Golestan province of Iran. An experiment was conducted in Golestan, Iran, from 1998 to 2000, to study the biology of the alder leaf beetle, Galerucella lineola, an important pest of alder, Alnus subcordata. Adults and larval instars of the pest feed on leaves of the host tr...

Conrad, H.R.; Hibbs, J.W.; Pratt, A.D., 1967: Effect of plane of nutrition and source of nitrogen on methionine synthesis in cows. A study was made to evaluate the effects of level of feed intake, ration composition and source of nitrogen on the synthesis of methionine in the rumen. The most significant effects on ruminal synthesis of essential amino acids were level of dry m...

Kanodia, K.; Vanikar, A.; Patel, R.; Suthar, K.; Nigam, L.; Kute, V.; Trivedi, H., 2015: Membranous Nephropathy With MPO-ANCA-Associated Crescentic GN. Antineutrophil cytoplasmic antibodies (ANCA)-associated glomerulonephritis (GN) is characterized by necrotizing and crescentic GN with paucity of immunoglobulin (Ig) and complement deposition, which is also known as pauci-immune crescentic GN. Mem...

Zannos Mariolea L.; Thomaidis T.; Georgizas G.; Gavrielidou E.; Benetos S., 1968: Diagnostic problems in severe neo natal jaundice and enz glucose 6 phosphate dehydrogenase deficiency in greece. Archives of Disease in Childhood 43(227): 36-41

Sion-Sarid, R.; Cohen, J.; Houri, Z.; Singer, P., 2014: Indirect calorimetry: a guide for optimizing nutritional support in the critically ill child. The metabolic response of critically ill children is characterized by an increase in resting energy expenditure and metabolism, and energy needs of the critically ill child are dynamic, changing from a hypermetabolic to hypometabolic state through...

Jespersen, S.Nørhøj.; Lundell, H.; Sønderby, C.Kaae.; Dyrby, T.B., 2014: Orientationally invariant metrics of apparent compartment eccentricity from double pulsed field gradient diffusion experiments. Pulsed field gradient diffusion sequences (PFG) with multiple diffusion encoding blocks have been indicated to offer new microstructural tissue information, such as the ability to detect nonspherical compartment shapes in macroscopically isotropic...

Akin, D.E., 1983: Structural characteristics limiting digestion of forage fiber. Cell walls of forage were studied for structural and chemical factors that limit digestion by rumen microorganisms using histochemistry, electron microscopy and anaerobic culturing of rumen populations on specific substrates. Certain tissues, such...