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Isolation and characterization of the LEU2 gene of Hansenula polymorpha

, : Isolation and characterization of the LEU2 gene of Hansenula polymorpha. Yeast 10(4): 509-513

A DNA fragment carrying the LEU2 gene of methylotrophic yeast Hansenula polymorpha was isolated by complementation of the leuB mutation of Escherichia coli. The nucleotide sequence of the isolated DNA fragment contains an open reading frame of 363 codons, coding for a protein 80% identical to the LEU2 gene product of Saccharomyces cerevisiae. Further downstream, there is a partial reading frame with no obvious similarity to known proteins. The LEU2 gene of H. polymorpha cannot complement the leu1 mutation of S. cerevisiae. The sequence has been entered in the EMBL data library under the Accession Number U00889.


PMID: 7941737

DOI: 10.1002/yea.320100410

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