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Isolation and characterization of the glucose-6-phosphate dehydrogenase encoding gene (gsdA) from Aspergillus niger

, : Isolation and characterization of the glucose-6-phosphate dehydrogenase encoding gene (gsdA) from Aspergillus niger. Molecular & General Genetics 247(2): 229-239

Genomic and cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) were isolated from the fungus Aspergillus niger. Sequence analysis of the glucose-6-phosphate dehydrogenase gene (gsdA) revealed an open reading frame of 1530 bp, encoding a protein of 58.951 kDa. The gsdA gene is interrupted by nine introns the most proximal of which is exceptionally large (348 bp). The region upstream of the ATG contains several C+T-rich stretches. The two major and one minor transcription start points are all located within these regions. In the upstream region several direct and inverted repeats, but no clear TATA or CCAAT boxes can be found. A. niger strains overproducing G6PD were constructed by cotransformation of gsdA subclones. Over-expression of G6PD was shown to be deleterious for the fungus especially when cotransformants were grown on media containing ammonia. Attempts to construct a gsdA null mutant by gene disruption were unsuccessful.


PMID: 7753033

Other references

Nikitina, L.A.; Buznikov, G.A., 1996: Serotonin inhibits maturation of the green toad oocytes induced by phorbol ether. The activator of protein kinase C phorbol-12-myristat-13-acetate (PMA) induces maturation of the definitive intact (coated with the follicular envelopes) and defolliculated oocytes of the green toad. This effect is more pronounced in case of defol...

Soriano, V.; Tor, J., 1990: The evidence for HTLV-1 infection in different risk groups in Barcelona. Medicina Clinica 95(14): 559-559

Lund, I.; Stovner, J., 1969: Dose-Response curves for tubocurarine, alcuronium and pancuronium. Acta Anaesthesiologica Scandinavica. Supplementum 37: 238-242

Lee, H.; Noh, J-Yeon.; Oh, Y.; Kim, Y.; Chang, J-Woong.; Chung, C-Woong.; Lee, S-Tae.; Kim, M.; Ryu, H.; Jung, Y-Keun., 2012: IRE1 plays an essential role in ER stress-mediated aggregation of mutant huntingtin via the inhibition of autophagy flux. Huntington's disease (HD), an inherited neurodegenerative disorder, is caused by an expansion of cytosine-adenine-guanine repeats in the huntingtin gene. The aggregation of mutant huntingtin (mtHTT) and striatal cell loss are representative f...

Fischer, Z.; Das, R.; Shipman, A.; Fan, J-Yuan.; Pence, L.; Bouyain, S.; Dobens, L.L., 2017: A iDrosophila model of insulin resistance associated with the human Trib3 Q/R polymorphism. Members of the Tribbles family of proteins are conserved pseudokinases with diverse roles in cell growth and proliferation. Both <i>Drosophila</i> Tribbles (Trbl) and vertebrate Trib3 proteins bind to Akt kinase to block its phosphoryl...

Saad Magalhães, C.; de Souza Medeiros, P.B.; Oliveira-Sato, J.; Custódio-Domingues, M.A., 2011: Clinical presentation and salivary gland histopathology of paediatric primary Sjögren's syndrome. Explore the presentation, diagnostic criteria and exocrine gland histopathology of paediatric primary Sjögren's syndrome (PPSjS). A case series of 8 children is reported and American-European Consensus Group (AECG-2002) criteria were examine...

Brusati, R., 1975: Glabello-maxillary suspension in the treatment of jaw fractures. Odontostomatologia E Implantoprotesi: 81-84

B.Soc Res Ageing, 1973: Meeting of the british society for research on aging london england january 24 1973. Age & Ageing 2(2): 128-129

Feldman, P.D.; Moises, H.C., 1988: Electrophysiological evidence for a1- and a2-adrenoceptors in solitary tract nucleus. American Journal of Physiology 254: 56-62

Herrmann, J., 1972: New methods for the evaluation and the interpretation of organoleptic qualities of foodstuffs and for the forecast of their changes. 5. organoleptic relative scales for sucrose and common salt concentrations, and calculation of the relative sweetness of sweetening agents. Nahrung 6 (3) 215-233