+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Localisation and properties of AMPA-insensitive kainate sites: Receptor autoradiography and gene expression in rat brain

, : Localisation and properties of AMPA-insensitive kainate sites: Receptor autoradiography and gene expression in rat brain. Neuroscience Letters 204(1-2): 121-124

Kainic acid (KA)-sensitive glutamate sites have been investigated by receptor autoradiography and in situ hybridisation histochemistry (ISHH) to evaluate their relationship to specific high-affinity KA receptors identified in molecular biological studies. Autoradiography with [3H]KA in the presence of the AMPA-selective antagonist NBQX (1 microM) revealed a widespread distribution of receptors through brain, especially in neocortex, hippocampal CA3, corpus striatum and granule cell layer of cerebellum. Specific binding was insensitive to the AMPA-selective agonist, S-5-fluorowillardiine, but inhibited by kainoids in a manner suggestive of receptor heterogeneity. Expression of the KA-2 receptor subunit mRNA by ISHH was also localised in hippocampal CA3 and cerebellar granule cells, suggesting some high-affinity native KA receptors labelled by [3H]KA were likely to include the KA-2 subunit in their heteromeric assembly.


PMID: 8929993

DOI: 10.1016/0304-3940(96)12335-1

Other references

Marsden, P.D.; Badaro, R.; Netto, E.M.; Casler, J.D., 1991: Spontaneous clinical resolution without specific treatment in mucosal leishmaniasis. Two cases of spontaneous arrest of disease progression without treatment are reported. One patient had 2 leishmanial skin ulcers which had healed uneventfully after treatment. A persistently positive ELISA and an ulcerative leishmaniasis skin test...

Tasch, P., 1968: A Permian trace fossil from the Antarctic Ohio Range. Transactions of the Kansas Academy of Science, 71: 33-37

Knolle, F., 1963: Brutvorkommen und Bestandsentwicklung des Graureihers (Ardea cinerea L.) im Braunschweiger Land wahrend der letzten hundert Jahre. Beitrk. Niedersachs: 61-66

Hagedorn, F.; Steiner, K.G.; Sekayange, L.; Zech, W., 1997: Effect of rainfall pattern on nitrogen mineralization and leaching in a green manure experiment in South Rwanda. The effects of green manures, sorghum residues and farmyard manure on N dynamics and crop yields were studied during three dry and wet seasons on a Typic Sombriudox in South Rwanda. In addition, a resin core study was conducted within a 4-year gre...

Raetzsch, C.F.; Brooks, N.L.; Alderman, J.McKee.; Moore, K.S.; Hosick, P.A.; Klebanov, S.; Akira, S.; Bear, J.E.; Baldwin, A.S.; Mackman, N.; Combs, T.P., 2009: Lipopolysaccharide inhibition of glucose production through the Toll-like receptor-4, myeloid differentiation factor 88, and nuclear factor kappa b pathway. Acute exposure to lipopolysaccharide (LPS) can cause hypoglycemia and insulin resistance; the underlying mechanisms, however, are unclear. We set out to determine whether insulin resistance is linked to hypoglycemia through Toll-like receptor-4 (T...

Tang, K-Chun.; Rury, A.; Orozco, M.B.; Egendorf, J.; Spears, K.G.; Sension, R.J., 2012: Ultrafast electrocyclic ring opening of 7-dehydrocholesterol in solution: the influence of solvent on excited state dynamics. Broadband UV-visible femtosecond transient absorption spectroscopy and steady-state integrated fluorescence were used to study the excited state dynamics of 7-dehydrocholesterol (provitamin D(3), DHC) in solution following excitation at 266 nm. Th...

Takatsuki, S.; Nemoto, S.; Sasaki, K.; Maitani, T., 2004: Production of acrylamide in agricultural products by cooking. Model studies were performed using various agricultural products to clarify the relation between cooking conditions and production of acrylamide (AA). Disc chips made from dried mashed potatoes, maize meal, wheat flour, rice flour (jyohshin-ko), g...

Duerler, T.S.; Maskarinec, G.G., 2010: Public health in the Republic of Palau: what we can learn from a small island country. The Republic of Palau has a well-run public health system despite being a small, relatively isolated island country with limited resources and limited funds. Using interviews with key informants, participant observation in clinics throughout Palau...

Li, W.; Zhao, K.; Kirberger, M.; Liao, W.; Yan, Y., 2016: Next generation sequencing technologies in cancer diagnostics and therapeutics: A mini review. The development of advanced molecular technologies has ushered in the era of 'omics' science, including transcriptomics, proteomics, and genomics. Genomics, or whole genome approach, has become the most comprehensive investigative method...

Hughes, E.G., 1992: Meta-analysis and the critical appraisal of infertility literature. Fertility and Sterility 57(2): 275-277