geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Long-term heart preservation by the combined method of simple immersion and coronary perfusion


, : Long-term heart preservation by the combined method of simple immersion and coronary perfusion. Journal of Heart and Lung Transplantation 15(3): 269-274

Background: The combined method of cold storage and coronary perfusion for prolonged preservation of donor hearts was evaluated through preservation and transplantation with the use of 10 pairs of adult mongrel dogs. Methods: In situ initial flush for cooling and coronary vascular washout was performed with a University of Wisconsin solution. The heart was then removed and immersed into a cold (4 degree C) University of Wisconsin solution. After 12-hour cold storage, 1-hour preservation was added by coronary perfusion using a 4 degree C oxygenated University of Wisconsin solution. High-energy phosphate (phosphocreatine, beta-adenosine triphosphate) and inorganic phosphate were measured by 31P-nuclear magnetic resonance spectroscopy at 0-hour cold storage, 12-hour cold storage, and immediately after coronary perfusion. Results: Phosphocreatine and beta-adenosine triphosphate decreased to 12.1% +- 24.2% (mean +- standard deviation), 37.4% +- 25.0%, respectively, after 12-hour cold storage, and significantly increased to 94.0% +- 48.7% (p lt 0.001), 48.8% +- 25.8% (p lt 0.05), respectively after 1-hour coronary perfusion. Disorder of nuclear arrangement and edema of muscular cells, which were observed after cold storage, were histologically restored after 1-hour coronary perfusion. After transplantation of preserved grafts, left ventricular pressure and left ventricular rate of pressure rise were evaluated in the graft with 12-hour cold storage only (group A) and in the graft with 12-hour cold storage and 1-hour coronary perfusion (group B). Left ventricular pressure 2 hours after transplantation in group B recovered to 76.1% significantly (p lt 0.01), compared with 51.9% in group A. Significantly higher values of left ventricular rate of pressure rise 2 hours after transplantation was observed in group B (83.0% +- 12.7%) compared with group A (68.2% +- 12.5%). Conclusions: These results indicate that the combined method of cold storage and coronary perfusion may be effective for myocardial protection during long-term preservation.

US$29.90

PMID: 8777210


Other references

Kurtz, V.E., 1975: Franconian upper cambrian trilobite faunas from the elvins group of southeast missouri usa. Franconian (Upper Cambrian) seas transgressing northwestward (present coordinates) from the proto-Atlantic seaway across the shelf, encountered a mature topography in the region southeast of the present St. Francois Mountains. Continued submergenc...

Mugwanya, K.K.; Baeten, J.M.; Wyatt, C.; Mugo, N.R.; Celum, C.L.; Ronald, A.; Kiarie, J.; Katabira, E.; Heffron, R., 2017: Frequency of monitoring kidney function in HIV-uninfected persons using daily oral tenofovir disoproxil fumarate pre-exposure prophylaxis. Wide-scale implementation of oral tenofovir-based pre-exposure prophylaxis (PrEP) for HIV prevention is now policy in many settings. However, the optimal frequency for monitoring kidney function remains uncertain. We investigated the impact of 6-m...

Anonymous, 1969: Mr. Munro serves up a challenge. Journal of the Canadian Dental Association 35(8): 391-392

Hosek, K.; Stĕpánek, L.; Stĕpánková, J., 1971: Motivation of potential fluctuation of health manpower in the Most area. Ceskoslovenske Zdravotnictvi 19(7): 279-283

Kaltsas, G.A.; Cunningham, J.L.; Falkmer, S.E.; Grimelius, L.; Tsolakis, A.V., 2011: Expression of connective tissue growth factor and IGF1 in normal and neoplastic gastrointestinal neuroendocrine cells and their clinico-pathological significance. Connective tissue growth factor (CTGF) and IGF1 are both expressed in a variety of tumours and are involved in tumourigenesis. However, information about their expression in the gastrointestinal (GI) neuroendocrine (NE) cells and tumours is mainly...

Anonymous, 2013: Organic fertilization An alternative to produce jalape o pepper under greenhouse conditions. The experiment was conducted during fall-winter seasons of years 2011-2012 to determine the effects of VCT on growth and productivity of “Hechicero” jalapeño pepper plants grown under organic and synthetic fertilization in green...

Abdullaeva, T.M., 1967: The effect of physiologically active substances on root formation and pigment content in leaf cuttings. Leaf cuttings of Saponaria officinalis, before setting in sand, were treated for 8 hours with different concentrations of heteroauxin, gibberellin, kinetin or kinin and 8-azaguanine. All the growth substances retarded the rate of root formation co...

Nie, X.; Liu, W.; Zhang, L.; Liu, Q., 2017: Genotoxicity of drinking water treated with different disinfectants and effects of disinfection conditions detected by umu-test. The genotoxicity of drinking water treated with 6 disinfection methods and the effects of disinfection conditions were investigated using the umu-test. The pretreatment procedure of samples for the umu-test was optimized for drinking water analysi...

Ansari, ZA.; Chatterji, A.; Parulekar, AH., 1986: Growth and production of benthic bivalve, Gafrarium pectinatum (Linn.) from west coast of India. Production of G. pectinatum was characterized by the presence of 0, 1 and 2 y age groups. Average monthly growth in length varied from 0.6 to 3 mm. The relationship between length and weight was isometric. The average biomass was 0.21 g (dry wt) m...

Camargo, C.de.A., 1968: Team work on the nursing unit. Revista Brasileira de Enfermagem 21(1): 57-63