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Long-term maintenance of functional rat hepatocytes in primary culture by additions of pyruvate and various hormones


, : Long-term maintenance of functional rat hepatocytes in primary culture by additions of pyruvate and various hormones. Biochimica Et Biophysica Acta. 1243(3): 329-335

Mature adult rat hepatocytes were cultured as monolayers in serum-free Williams medium E containing 10-7 M each of insulin (Ins), dexamethasone (Dex) and triiodothyronine (T-3) and 30 mM pyruvate. The hepatocytes remained morphologically intact for at least 14 days, during which period they maintained normal liver functions such as the expressions of cytochrome P-450 mRNA and glucokinase and secretion of albumin. They also retained the ability to resume proliferation. Cells cultured with pyruvate had a much higher ATP level than those without pyruvate, suggesting that pyruvate can sustain functional hepatocytes for a long period in culture in the presence of Ins, Dex and T-3, probably by producing enough energy for their maintenance.

US$19.90

PMID: 7727507

DOI: 10.1016/0304-4165(94)00155-q


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