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Low-level electrochemical detection of glucose oxidase and a glucose oxidase conjugate

, : Low-level electrochemical detection of glucose oxidase and a glucose oxidase conjugate. Biosensors & Bioelectronics. 10(6-7): 621-631

An amperometric sensor for glucose oxidase is described. Partial optimization of the working electrode potential at 0.4 V vs. Ag/AgCl, 0.1 M KCl at a glucose concentration of 30 mM allows a low detection limit of 50 pM glucose oxidase within 25 min. The sensor exhibits a high degree of precision and reproducibility, and the same electrode may be re-used for many successive quantitative determinations. A mechanism for the sensor is proposed, in which hydrogen peroxide generated by glucose oxidase in bulk solution is oxidized at the working electrode, generating a current response that can be related to the glucose oxidase concentration. Conjugation of glucose oxidase with a coumarin-based pesticide analogue does not affect the amperometric response.


DOI: 10.1016/0956-5663(95)96938-u

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