+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Membrane-mediated release of nucleotide from an initiator of chromosomal replication, Escherichia coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer

, : Membrane-mediated release of nucleotide from an initiator of chromosomal replication, Escherichia coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer. Journal of Biological Chemistry 273(9): 5167-5173

DnaA protein, the initiator protein of E. coli chromosomal replication, can be rejuvenated from an inactive ADP form to active ATP-DnaA protein by acidic phospholipids in a fluid bilayer. Cross-linking studies with the photoactivable phospholipid analog 1-O-hexadecanoyl-2-O-[9-[[[2-[125I]iodo-4-(trifluoromethyl-3H- diazirin -3-yl)benzyl]oxy]carbonyl]nonanoyl]-sn-glycero-3-phosphocholine reveal insertion of DnaA protein into the hydrophobic region of the bilayer; this insertion is accompanied by membrane-mediated dissociation of the tightly bound allosteric nucleotides ADP and ATP. Photolabeling of DnaA protein occurred with membrane properties that resembled those needed for reactivation of ADP-DnaA protein; efficient labeling of DnaA protein was observed only when the lipid analog was incorporated into anionic vesicles and the temperature during treatment was above the gel to liquid crystalline phase transition. Predominant hydrophobic photolabeling was localized within a single region of DnaA protein, a region that contains putative amphipathic helices and has been shown to contain information essential for functional interaction with membranes.


PMID: 9478970

DOI: 10.1074/jbc.273.9.5167

Other references

Matsubara, K.; Abe, E.; Ochi, H.; Kusanagi, Y.; Ito, M., 2003: Changes in serum concentrations of tumor necrosis factor alpha and adhesion molecules in normal pregnant women and those with pregnancy-induced hypertension. To study whether serum tumor necrosis factor alpha gene (TNFalpha) and adhesion molecule levels are indicators of the onset of pregnancy-induced hypertension (PIH), we compared levels of these molecules between normal pregnant women and PIH patien...

Muller, D., 1985: Potamopyrgus jenkinsi (E.A. Smith) im Starnberger See. Heldia, 13: 107

Elinder, L.Schäfer.; Bergström, H.; Hagberg, J.; Wihlman, U.; Hagströmer, M., 2011: Promoting a healthy diet and physical activity in adults with intellectual disabilities living in community residences: design and evaluation of a cluster-randomized intervention. Many adults with intellectual disabilities have poor dietary habits, low physical activity and weight disturbances. This study protocol describes the design and evaluation of a health intervention aiming to improve diet and physical activity in th...

Milne, S.A.; Critchley, H.O.D.; Drudy, T.A.; Baird, D.T., 2000: Androgen and oestrogen receptor expression in early pregnancy decidua after exposure to mifepristone. Journal of Reproduction & Fertility Abstract Series (25): 31, July

Averkiev, B.B.; Zubarev, D.Yu.; Wang, L-Ming.; Huang, W.; Wang, L-Sheng.; Boldyrev, A.I., 2008: Carbon avoids hypercoordination in CB6(-), CB6(2-), and C2B5(-) planar carbon-boron clusters. The structures and bonding of CB6-, C2B5-, and CB62- are investigated by photoelectron spectroscopy and ab initio calculations. It is shown that the global minimum structures for these systems are distorted heptacyclic structures. The previously r...

Kingma, M.J., 1978: Ligament injuries. Nederlands Tijdschrift Voor Geneeskunde 122(42): 1619-1620

Wang, L.H.; Tawil, R., 2016: Facioscapulohumeral Dystrophy. Facioscapulohumeral muscular dystrophy (FSHD) is a clinically recognizable and relatively common muscular dystrophy. It is inherited mostly as an autosomal dominant disease or in a minority of cases, in a digenic pattern. The disease manifestation...

Hlava P.F.; Prinz Martin; Keil Klaus, 1972: Niobian rutile in an Apollo 14 KREEP fragment. Electron microprobe analysis, structural formula.

Kim, H.; Oh, J.Kyu.; Kim, M.Ki.; Bae, K.; Choi, H., 2017: Reduced antibiotic prescription rates following physician-targeted interventions in a dental practice. The prescription rate for antibiotics in dental clinics is not declining despite the increase in the antibiotic resistance problem. In this study, we observed the change in antibiotic prescription rates by dentists in a Korean dental hospital for...

Wert K.L., 1980: Intra molecular friedel crafts alkylations 2 an efficient synthesis of biologically active 1 2 3 4 tetra hydro iso quinolines. Tetrahedron Letters: 1393-1396