+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Microwave digestion methods for the determination of trace elements in brain and liver samples by inductively coupled plasma mass spectrometry

, : Microwave digestion methods for the determination of trace elements in brain and liver samples by inductively coupled plasma mass spectrometry. Analytical and Bioanalytical Chemistry 355(2): 120-128

Two microwave digestion systems (open-focused and closed-pressurized) were tested for the mineralization of human brain and bovine liver (NIST SRM 1577a) as dissolution steps prior to the determination of 16 trace elements (Bi, Cd, Co, Cs, Cu, Fe, Hg, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Tl, and Zn) by inductively coupled plasma mass spectrometry (ICP-MS). Digestion parameters (mass of sample, digestion mixture, and power/time steps) were optimized using temperature and pressure sensors. Digestions with the open-focused microwave system require larger volumes of conc. HNO(3) and 30% H(2)O(2) than digestions with the closed-pressurized system. Both systems produce correct results for the bovine liver samples. The concentrations obtained for the digests of the open-focused system tend to be less precise than the concentrations from the "closed-pressurized" digests. Because the "open-focused" digests must be diluted to 50 mL to bring the acid concentration to 0.7-2.0 mol/L required by the ICP-MS (closed-pressurized digests need to be diluted to only 20 mL), the detection limits for the system with the open-focused digestion are higher than for the system with the closed-pressurized digestor. The open-focused digestor cannot handle more than 150 mg brain tissue, whereas the closed-pressurized system can mineralize 470 mg. The latter method gave better results with brain tissue than the open-focused system. The preparation of brain tissue as reference material for the determination of trace elements in brain samples is described.


PMID: 15045434

DOI: 10.1007/s0021663550120

Other references

Egerter, D.A.; Beckstead, J.H., 1988: Malignant lymphomas in the acquired immunodeficiency syndrome. Additional evidence for a B-cell origin. An increased incidence of high-grade malignant non-Hodgkin's lymphomas has been reported in homosexual men. This phenomenon appears to represent another facet of the acquired immunodeficiency syndrome (AIDS). Histologically, the majority of t...

Liao, Y.; Huang, Z.; Huh, J.; Pentz, M.Ann.; Chou, C-Ping., 2013: Changes in friends' and parental influences on cigarette smoking from early through late adolescence. This study examined the changes in friends and parental influences on cigarette smoking across two developmentally distinct social environments for adolescents: junior high school and high school. Longitudinal data consisting of seven repeated mea...

Fischer, W.; Kraatz, H., 1977: The 75th birthday of Helmut Kraatz, Chief Editor of Zentralblatt für Gynekologie. Ceskoslovenska Gynekologie 42(9): 716-717

Anonymous, 1981: Kewaunee scientific equipment corporation. Analytical Chemistry 53(9): 1046a-1046a

Izard, C., 1957: Studies of the physiological action of nicotine. I. Growth inhibition in the presence of nicotine and other inhibitors. Nicotine reduced the growth rate of oat coleoptiles; increasing the pH in the presence of nicotine had the same effect. Nicotine reduced the growth-stimulating effect of indole beta -acetic acid but augmented the inhibiting effect of sodium fluori...

Santoso, Y.; Hwang, L.Chin.; L.R.ste, L.; Kapanidis, A.N., 2008: Red light, green light: probing single molecules using alternating-laser excitation. Single-molecule fluorescence methods, particularly single-molecule FRET (fluorescence resonance energy transfer), have provided novel insights into the structure, interactions and dynamics of biological systems. ALEX (alternating-laser excitation...

Kang, Y-Ju.; Yang, S-Jip.; Park, G.; Cho, B.; Min, C-Ki.; Kim, T-Yoon.; Lee, J-Sung.; Oh, I-Hoan., 2007: A novel function of interleukin-10 promoting self-renewal of hematopoietic stem cells. Self-renewal of hematopoietic stem cells (HSCs) is key to their reconstituting ability, but the factors regulating the process remain poorly understood. Here, we show that Interleukin-10 (IL-10), a pleiotropic immune modulating cytokine, can also...

Megias, M.D.; Cherney, J.H.; Cherney, D.J.R., 1997: Effects of phenolic compounds in cell walls of orange and artichoke by-product silages on in vitro digestibility. By-products of the canning industry are extensively used as ruminant fodder in some areas of the world. Characterization of the phenolic content of these feeds and their influence on digestibility is lacking. Phenolic carbohydrate complexes were i...

Bias, P.; Buchner, A.; Klesser, B.; Laufer, S., 2004: The gastrointestinal tolerability of the LOX/COX inhibitor, licofelone, is similar to placebo and superior to naproxen therapy in healthy volunteers: results from a randomized, controlled trial. OBJECTIVE: Concerns exist over the safety of conventional nonsteroidal anti inflammatory drugs (NSAIDs) and selective cyclooxygenase (COX)-2 inhibitors. In experimental models, licofelone, a competitive inhibitor of 5-lipoxygenase (5-LOX) and COX-...

Ribeiro A.S.R.; Machado M.O.; Silveira P.Jr; Zonta E.P., 1985: The effects of irrigation and nitrogenated fertilizer on the occurrence and control of rice blast. Fitopatologia Brasileira 10(2): 296