+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Model of human transitional cell carcinoma: tumor xenografts in upper urinary tract of nude rat

, : Model of human transitional cell carcinoma: tumor xenografts in upper urinary tract of nude rat. Journal of Endourology 9(1): 1-7

An in vivo model for the study of human transitional cell carcinoma (TCC) in the urinary tract is desirable. Orthotopic xenografts are useful in order to approximate better the behavior of human tumor cells in situ. Prior models have been described in the urinary bladder of the nude mouse and rat. We have developed the first model of implantation of human TCC in the upper urinary tract of an experimental animal. The kidneys of homozygous nude rats 4 weeks of age were inoculated With 1-5 times 10-6 cells of the RT4 well-differentiated papillary human TCC line through bilateral flank incisions, with transparenchymal injection of tumor cells into either the collecting system (renal pelvis) or the parenchyma. The overall implantation rate was 92% (54/59 kidneys). However, implantation into the collecting system occurred in only 45% (18/45) of the group. Ligation of the ureter prior to inoculation to produce urine stasis improved the mucosal implantation rate. Despite suspect urine cytology findings in the renal pelvis injection group, no distal seeding of the urinary tract was seen. Intraparenchymal tumor growth was less differentiated and had a higher fraction of mitotic cells than mucosal tumors. Vascular and lymphatic invasion were commonly seen; however, distant metastasis was not observed. This model will prove useful in determining the role of seeding in recurrent disease and in developing less invasive modalities for the treatment of upper tract TCC.


PMID: 7780425

DOI: 10.1089/end.1995.9.1

Other references

Hsu, C-Chung.; Chen, W-Ming.; Chen, S-Ru.; Tseng, Y-Ting.; Lin, P-Chu., 2015: Effectiveness of Music Listening in Patients With Total Knee Replacement During CPM Rehabilitation. This study investigated the effects of music listening on the anxiety, heart rate variability (HRV), and joint range of motion (ROM) of patients undergoing continuous passive motion (CPM) after total knee replacement surgery. An experimental desig...

Yadav, BE., 1994: The scolopendrid centipedes. Science and Culture. July-December; 607-12: 77-79

Affeltranger, C.E.; Burns, E.B., 1983: Root rots on Shumard oak in a central Louisiana nursery. Isolations from apparently diseased 1-0 Quercus shumardii seedlings grown at a small private nursery yielded Cylindrocladium scoparium and a Phytophthora sp., the first report of the former on this host and in La.

Cebe, P., 1981: Lindane in France, the United States and in the world. Phytoma (327) 24-26

Goldsmith J.R.; Potashnik G.; Israeli R., 1984: Reproductive outcomes in families of 1 2 di bromo 3 chloro propane exposed men. The families of 30 workers employed in a plant which, until 1977, had produced 1,2-dibromo-3-chloropropane (DBCP), were monitored for 5 yr. Thirteen men who were azospermic in 1977 had > 100 h of estimated exposure in production; of these, 4 ha...

Wodon C., 1982: Separation of main purine nucleotides and some synthetic analogs by simple chromatography on an anion exchange resin. Journal of Chromatography 246(2): 327-333

Aagaard, P.; Simonsen, E.B.; Andersen, J.L.; Magnusson, S.P.; Halkjaer Kristensen, J.; Dyhre Poulsen, P., 1999: Increased contractile RFD and neuromuscular activation induced by heavy-resistance strength training. Medicine & Science in Sports & Exercise 31(5 SUPPL ): S115, May

Morant, M.A.; Warren, H.L.; Von Qualen, S.K., 1993: A synthetic medium for mass production of pycnidiospores of Stenocarpella species. Sporulation and vegetative growth of Stenocarpella maydis and S. macrospora were evaluated by growing the organisms in basal mineral salts medium amended with sucrose (0-30 g/L) and biotin (0-50 mg/L) for 28 days at 23 C under constant illuminatio...

Jacobson, L.K.; Dutton, G.N., 2000: Periventricular leukomalacia: an important cause of visual and ocular motility dysfunction in children. The immature visual system in infants born preterm is vulnerable to adverse events during the perinatal period. Periventricular leukomalacia affecting the optic radiation has now become the principal cause of visual impairment and dysfunction in c...

Navarrete, M.H.; Bernabeu, C., 1978: Soluble polypeptides from meristematic and mature cells of Allium cepa L. roots. By using electrophoresis on SDS [sodium dodecyl sulfate] polyacrylamide gels, the soluble protein fraction from a section of the meristem, where most cells are in the division last cycle, and from the mature region of A. cepa L. roots were studied...