+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular basis of estrogen regulation of Hageman factor XII gene expression

, : Molecular basis of estrogen regulation of Hageman factor XII gene expression. Endocrinology 136(11): 5076-5083

Estrogen therapy has been reported to cause multiple alterations in hemostasis and to increase blood levels of several procoagulants, including Hageman factor (factor XII (FXII)). Liver FXII gene expression has been investigated in ovariectomized rats, treated or not with 17-beta-estradiol, A 6-fold stimulation of FXII gene transcription was observed in treated compared to untreated animals, indicating that 17-beta-estradiol is able to induce FXII gene expression in vivo. We have recently shown that human FXII promoter contains an imperfect palindrome, 5'-GGGCAnnnTGACC-3', at position -43/-31 resembling the consensus estrogen-responsive element (ERE). Portions of different length of the FXII promoter were fused to the chloramphenicol acetyltransferase (CAT) coding sequence and transiently cotransfected with human estrogen receptor (ER) into NIH3T3 and HepG2 cells in the presence or absence of 17-beta-estradiol. A 230-base pair fragment of FXII promoter, spanning nucleotides - 181/49, conferred a strong estrogen responsiveness to the CAT reporter gene, suggesting that a functional ERE resides in this region. Cognate receptors, such as those for thyroid hormone or retinoic acid, did not stimulate CAT activity. Gel mobility assays demonstrated a specific interaction between ER and the 230-bp FXII promoter fragment containing the putative ERE palindrome. Similar results were obtained when an oligonucleotide spanning the consensus ERE was used; the complex between ER and FXII promoter sequences was supershifted after the addition of an anti-ER monoclonal antibody. Insertion of FXII-ERE into the heterologous thymidine kinase promoter conferred a strong estrogen responsiveness that was abolished by mutations of the 5'-half of the palindrome. These results represent the first demonstration at the molecular level of the regulation of a blood coagulation factor gene by 17-beta-estradiol as well as the first identification of a functional ERE within this class of genes.


PMID: 7588244

DOI: 10.1210/endo.136.11.7588244

Other references

Aponte G.W.; Garcia R.; Taylor I.L., 1986: Distribution and release of peptide yy in the rat. Gastroenterology 90(5 PART 2): 1329

Pardemann, G.; Magnus, S.; Heine, H.; Schmidt, H., 1971: Studies on peripheral blood vessels using the ultrasonic Doppler method. Das Deutsche Gesundheitswesen 26(52): 2441-2444

Kim, D-Yeon.; Islam, S.; Mondal, N.Taposh.; Mussell, F.; Rauchholz, M., 2011: Biventricular thrombi associated with peripartum cardiomyopathy. A 22-year old woman visited the LAMB Hospital, Parbatipur, Dinajpur, Bangladesh, in February 2010, with exertional dyspnea for three weeks. She had had a normal vaginal delivery four months ago; 2-dimensional echocardiogram showed severe left vent...

Anonymous, 1971: Care in handling mercury. School Dental Service Gazette, New Zealand 31: 58-58

Brand, H.J.; Hanekom, J.D.; Scheepers, D., 1988: Internal consistency of the Sport Competition Anxiety Test. The Sport Competition Anxiety Test was administered to 77 university athletes, 55 men and 22 women whose ages ranged from 18 to 27 yr., to ascertain internal consistency of responses to the questionnaire. Both methods used (Guttman and Kuder-Richa...

Fisher, B.L.; Cover, S.P., 2007: Ants of North America a guide to the genera. Unknown

Anonymous, 2011: Simultaneous Identification of FiveBifidobacteriumSpecies Isolated from Human Beings Using Multiple PCR Primers. On the basis of 16S rRNA sequences, 5 species-specific forward primers were designed for the identification of 5Bifidobacteriumspecies isolated from human intestine, namelyB. bifidum, B. adolescentis, B. infantis, B. breveandB. longum. As the 5 pr...

Scott, J.W.; Davis, L.M.; Shannon, D.E., 1995: Properties of odorants producing maximal responses in different parts of the rat olfactory epithelium. Chemical Senses 20(6): 775-776

Katarzyna Dymek; Petr Dejmek; Valentina Panarese; António A.V.cente; Lars Wadsö; Christine Finnie; Federico Gómez Galindo, 2012: Effect of pulsed electric field on the germination of barley seeds. This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEF). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages ( (control), 11, 16, 24, 32,...

Al-Fayez, S.F.; Salleh, M.; Ardawi, M.; Zahran, F.M., 1988: Effects of sheesha and cigarette smoking on pulmonary function of Saudi males and females. The effects of sheesha or cigarette smoking on pulmonary function values were examined in 441 and 154 male and female smokers respectively. Cigarette smokers showed a marked decline in pulmonary function values with increasing age that was more pr...