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Molecular characterization of the sodium channel subunits expressed in mammalian cerebellar Purkinje cells


, : Molecular characterization of the sodium channel subunits expressed in mammalian cerebellar Purkinje cells. Proceedings Of The National Academy Of Sciences Of The United States Of America. 94(13): 7059-7064

Inactivating and noninactivating Na+ conductances are known to generate, respectively, the rising phase and the prolonged plateau phase of cerebellar Purkinje cell (PC) action potentials. These conductances have different voltage activation levels, suggesting the possibility that two distinct types of ion channels are involved. Single Purkinje cell reverse transcription-PCR from guinea pig cerebellar slices identified two Na+ channel alpha subunit transcripts, the orthologs of RBI (rat brain I) and Nach6/Scn8a. The latter we shall name CerIII. In situ hybridization histochemistry in rat brain demonstrated broad CerIII expression at high levels in many neuronal groups in the brain and spinal cord, with little if any expression in white matter, or nerve tracts. RBII (rat brain II), the most commonly studied recombinant Na+ channel alpha subunit is not expressed in PCs. As the absence of Scn8a has been correlated with motor endplate disease (med), in which transient sodium currents are spared, RBI appears to be responsible for the transient sodium current in PC. Conversely, jolting mice with a mutated Scn8a message demonstrates PC abnormalities in rapid, simple spike generation, linking CerIII to the persistent sodium current.

US$19.90

PMID: 9192691

DOI: 10.2307/42278


Other references

Anonymous, 1969: Cvma hospital inspection. Canadian Veterinary Journal 10(3): 85-88

Tangendjaja, B.; Wina, E., 1999: Tannins and ruminant production in Indonesia. This paper reports on the composition of forage fed to animals in Indonesia and the importance of condensed tannins which may influence productivity. Ruminant livestock in Indonesia are fed almost exclusively on forages, by cut-and-carry systems o...

Yamanouchi, Y.; Brewer, J.E.; Mowrey, K.A.; Donohoo, A.M.; Wilkoff, B.L.; Tchou, P.J., 1998: Optimal small-capacitor biphasic waveform for external defibrillation: Influence of Phase-I tilt and Phase-2 voltage. Background-Biphasic waveforms have been reported to be more efficacious than monophasic waveforms for external defibrillation. This study examined the optimal phase-1 tilts and phase-2 leading-edge voltages with small capacitors (60 and 20 muF) fo...

Wurmbach, H., 1945: Modellversuch zur Bildung der Kollagenfeinstruktur des Knorpels. Zoologische Jahrbuecher Jena Anatomie, 39: 251-271

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