+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular genetic analysis of multi-drug resistance in Indian isolates of Mycobacterium tuberculosis

, : Molecular genetic analysis of multi-drug resistance in Indian isolates of Mycobacterium tuberculosis. Memorias do Instituto Oswaldo Cruz 93(5): 589-594

A total of 116 isolates from patients attending the out-patient department at the All India Institute of Medical Sciences, New Delhi and the New Delhi Tuberculosis Centre, New Delhi, India were collected. They were analyzed for resistance to drugs prescribed in the treatment for tuberculosis. The drug resistance was initially determined by microbiological techniques. The Bactec 460TB system was employed to determine the type and level of resistance in each isolate. The isolates were further characterized at molecular level. The multi-drug loci corresponding to rpo beta, gyr A, kat G were studied for mutation(s) by the polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) technique. The SSCP positive samples were sequenced to characterize the mutations in rpo beta, and gyr A loci. While previously reported mutations in the gyr A and rpo beta loci were found to be present, several novel mutations were also scored in the rpo beta locus. Interestingly, analysis of the gyr A locus showed the presence of point mutation(s) that could not be detected by PCR-SSCP. Furthermore, rifampicin resistance was found to be an important marker for checking multi-drug resistance (MDR) in clinical isolates of Mycobacterium tuberculosis. This is the first report on molecular genetic analysis of MDR tuberculosis from India, and highlights the increasing incidence of MDR in the Indian isolates of M. tuberculosis.


PMID: 9830523

Other references

Charnley, N.R.; Potts, P.J.; Long, J.V.P., 1994: Modelling the transmission of X-rays through glass capillary waveguides Implications for the design of a laboratory X-ray microprobe. Journal of Analytical Atomic Spectrometry 9(11): 1185-1193

Petrovicova, M.; Panic, M.; Jamriska, M.; Petrovic, M.; Panic, M., 1988: Heritability and repeatability of reproductive traits in sows. Data were obtained on 438 Swedish Landrace (SL) and 222 Large White X SL sows, and on their 2450 and 902 litters from 10 and 8 parities resp. For the SL sows born in 3 consecutive yr, the hsuperscript 2 of age at 1st conception was 0.103, 0.123 an...

WurenQiqige; Zhang,A.M.; WuXiaoDong, 2008: Study on the change of micro-relief and plant community in the Hulunbuir sandy nature meadow prairie. Multifunctional Grasslands In A Changing World, Volume 1: Xxi International Grassland Congress And Viii International Rangeland Congress, Hohhot, China, 29 E 5 Y: 3

Tiba, R.; Kosuge, S., 1985: North Pacific shells (16) genus Nipponotrophon Kuroda and Habe. Occasional Publication of the Institute of Malacology of Tokyo. March; 16: 1-10

Sim, R.J.; Matthew, S.; Foley, R.J.; Robinson, P.J., 2010: Initial outcomes from universal newborn hearing screening in Avon. The Avon Area Health Authority was a first-phase site for introduction of universal newborn screening in the UK. The aims of this study were: to review the programme's results to date; to assess the impact screening would have on other servic...

Staff, A.Cathrine.; Redman, C.W.G.; Williams, D.; Leeson, P.; Moe, K.; Thilaganathan, B.; Magnus, P.; Steegers, E.A.P.; Tsigas, E.Z.; Ness, R.B.; Myatt, L.; Poston, L.; Roberts, J.M.; Staff, A.Cathrine.; Redman, C.W.G.; Magnus, P.; Steegers, E.A.P.; Tsigas, E.Z.; Myatt, L.; Poston, L.; Roberts, J.M., 2016: Pregnancy and Long-Term Maternal Cardiovascular Health: Progress Through Harmonization of Research Cohorts and Biobanks. Hypertension 67(2): 251-260

Bertelsen, E., 1970: Butter oil as basic fat for the food industry. After outlining uses of various fats in foods the author describes briefly continuous butter oil manufacture in an Alfa-Laval installation with a capacity of -1900 kg product/h, and discusses future prospects.

Vincent, LS., 1974: A method for rearing spiders. Bulletin Br arachnol Soc, 32: 52

Holusa, Otakar, 2000: Vysledky faunistickeho pruzkumu vazek (Odonata) na uzemi Sumavy. During the period 1997-1998 in Bohemian Forest 27 dragonflies species were found. Literary records of dragonflies on the territory of Bohemian Forest are summarised. The first record of Aeshna caerulea in the Bohemian Forest. Records of occurrence...

Jenkins, E., 2001: Facility profile. Retail shops inspire new facility. The Health & Wellness Center by Doylestown Hospital, Warrington, Pa. Health Facilities Management 14(10): 14-15