geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens


, : Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens 49(4): 403-413

Macrophages are widely distributed in most tissues of the body, where they play important roles in host defense and repair of tissue damage. In this report we describe the production and characterization of a panel of six monoclonal antibodies (mAb) against porcine macrophages and their use for phenotyping tissue macrophages. All mAbs were produced by immunizing mice with porcine alveolar macrophages. Three of them (2A 10/11, 3B11/11 and 3F7/11) react mainly with macrophages and, at a lower extent, blood monocytes, whereas the others (1E12/11, 2C12/10 and 4E9/11) also recognize granulocytes. Antigens recognized by these antibodies could be characterized by Western blot and/or immunoprecipitation, with the exception of that one recognized by 2C12/10. By their behavior in SDS-PAGE under reducing and nonreducing conditions, all seem to be single polypeptides, whose apparent molecular weight under reducing conditions are: 1E12/11 and 3B11/11 larger than 204 kDa; 2A 10/11, 150 kDa; 4E9/11, 125-170 kDa; and 3F7/11, 135 kDa. Immunohistochemical analyses of both lymphoid and non-lymphoid organs using these mAbs reveal important antigenic heterogeneity among tissue macrophages. These mAbs are, therefore, useful tools for the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions.

US$19.90

PMID: 9151393

DOI: 10.1111/j.1399-0039.1997.tb02769.x


Other references

Spinkyte Backaitiene, R., 2005: Habitat use by the wolf (Canis lupus L.) in North Lithuania. Habitat use by wolves was researched with the aim to understand their visiting frequency of different landscape sites, priorities for resting place selection and the influence of anthropogenic factors on wolves' activity. Snow tracking of wol...

Hill, DS., 1980: The Neritidae (Mollusca; Prosobranchia) of Hong Kong. Unknown

Baccetti, N.; Basso, M.; D.F.veri, A.; Talamelli, A., 1997: Resightings of greater flamingos in Tunisia, February 1994. WIWO Report, 54: 111-118

Guizy, M.; David, M.; Arias, C.; Zhang, L.; Cofan, M.; Ruiz-Gutierrez, V.; Ros, E.; Lillo, M.P.lar; Martens, J., R.; Valenzuela, C., 2008: Modulation of the atrial specific Kv1.5 channel by the n-3 polyunsaturated fatty acid, alpha-linolenic acid. Epidemiological, clinical and experimental studies suggest that the cardioprotective effect of fish intake is mainly due to the antiarrhythmic properties of marine n-3 polyunsaturated fatty acids (PUFA), which modulate ion currents. Emerging evid...

Broadhead Ronald F., 1993: Petrography and reservoir geology of Upper Devonian shales, northern Ohio. U S

D.G.ay; J.H.rding, 2012: Acid Mine Drainage Index AMDI a benthic invertebrate biotic index for assessing coal mining impacts in New Zealand streams. Acid mine drainage (AMD) is a widespread phenomenon globally. Drainage into streams from coal mines often contains a cocktail of acidic waters high in dissolved metals, and consequently stream invertebrate communities may be severely impacted. Tra...

Ghysen, A.; Dambly Chaudiere, C., 1995: The formation of sensory organs in Drosophila. M-S (Medecine Sciences) 11(2): 178-188

Hujoel, P.P.; Drangsholt, M.; Spiekerman, C.; DeRouen, T.A., 2002: Periodontitis-systemic disease associations in the presence of smoking--causal or coincidental?. Periodontology 2000 30: 51-60

Ben Dhaou, B.; Ketari, S.; Boussema, F.; Aydi, Z.; Baili, L.; Rokbani, L., 2013: Pulmonary aneurysms in Behçet's disease completely resolved after medical therapy. Pulmonary artery aneurysm in Behcet disease compromises seriously the diagnosis since its fatal rupture is still the major cause of death in this disease. Pulmonary artery aneurysm requires urgent management and several treatments have been propos...

Salvato B.; Zatta P., 1972: Research on hemo cyanins part 20 the effect of the removal of amino groups in the active site in hemo cyanin. Bollettino Societa Italiana Biologia Sperimentale 48(24): 1311-1312